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PMID: 1717823 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

AGP/EBP(LAP) expressed in rat hepatoma cells interacts with multiple promoter sites and is necessary for maximal glucocorticoid induction of the rat alpha-1 acid glycoprotein gene.

Molecular and cellular biology ·Vol. 11 ·No. 10 ·1991-10-00 ·Pages 4959-65

Williams P, Ratajczak T, Lee SC, Ringold GM

Abstract

Transcription of the rat alpha 1-acid glycoprotein (AGP) gene is induced by glucocorticoids. In addition to the glucocorticoid response element which maps to bases -120 to -107, sequences located between bases -106 to -42 have been shown to be necessary for hormone induction. We have previously identified multiple sites of C/EBP interaction with the AGP promoter in the region -106 to -64. In this study, we purify and identify a C/EBP family member, AGP/EBP(LAP), present in the rat hepatoma cell line HTC (JZ.1) which also binds to the C/EBP recognition sites in this region. Mutations in the recognition sites that prevent binding are analyzed, and the results suggest a positive as well as a possible inhibitory role for AGP/EBP(LAP) in the glucocorticoid induction of the gene in HTC (JZ.1) cells.

MeSH Terms
Animals Base Sequence Binding Sites/physiology Blotting, Western CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins/genetics,physiology Dexamethasone/pharmacology Gene Expression Regulation/drug effects Glucocorticoids/pharmacology Glycoprotein Hormones, alpha Subunit/genetics,physiology Liver Neoplasms, Experimental Molecular Sequence Data Mutation/physiology Nuclear Proteins/genetics,physiology Orosomucoid/genetics Promoter Regions, Genetic/physiology Rats Transcription Factors/genetics,physiology Tumor Cells, Cultured
Chemicals
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins Glucocorticoids Glycoprotein Hormones, alpha Subunit Nuclear Proteins Orosomucoid Transcription Factors Dexamethasone
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Williams P
Syntex Research, Palo Alto, California 94304.
Ratajczak T
Lee S C
Ringold G M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.10.4959-4965.1991
Published
1991-10-00
Pages
4959-65
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361476
Subset
IM
Grants
NIGMS NIH HHS · GM25821 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.10.4959-4965.1991 PMC Full Text View journal details
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