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PMID: 7896867 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Overexpression of wild-type and mutant ARF1 and ARF6: distinct perturbations of nonoverlapping membrane compartments.

The Journal of cell biology ·Vol. 128 ·No. 6 ·1995-03-00 ·Pages 1003-17

Peters PJ, Hsu VW, Ooi CE, Finazzi D, Teal SB, Oorschot V, Donaldson JG, Klausner RD

Abstract

The ARF GTP binding proteins are believed to function as regulators of membrane traffic in the secretory pathway. While the ARF1 protein has been shown in vitro to mediate the membrane interaction of the cytosolic coat proteins coatomer (COP1) and gamma-adaptin with the Golgi complex, the functions of the other ARF proteins have not been defined. Here, we show by transient transfection with epitope-tagged ARFs, that whereas ARF1 is localized to the Golgi complex and can be shown to affect predictably the assembly of COP1 and gamma-adaptin with Golgi membranes in cells, ARF6 is localized to the endosomal/plasma membrane system and has no effect on these Golgi-associated coat proteins. By immuno-electron microscopy, the wild-type ARF6 protein is observed along the plasma membrane and associated with endosomes, and overexpression of ARF6 does not appear to alter the morphology of the peripheral membrane system. In contrast, overexpression of ARF6 mutants predicted either to hydrolyze or bind GTP poorly shifts the distribution of ARF6 and affects the structure of the endocytic pathway. The GTP hydrolysis-defective mutant is localized to the plasma membrane and its overexpression results in a profound induction of extensive plasma membrane vaginations and a depletion of endosomes. Conversely, the GTP binding-defective ARF6 mutant is present exclusively in endosomal structures, and its overexpression results in a massive accumulation of coated endocytic structures.

MeSH Terms
ADP-Ribosylation Factor 1 ADP-Ribosylation Factors Amino Acid Sequence Animals Base Sequence Cell Compartmentation Cell Membrane/metabolism,ultrastructure Cells, Cultured DNA Primers GTP-Binding Proteins/biosynthesis,genetics Golgi Apparatus/metabolism,ultrastructure Haplorhini Humans Molecular Sequence Data Mutation Sequence Alignment
Chemicals
DNA Primers GTP-Binding Proteins ADP-Ribosylation Factor 1 ADP-Ribosylation Factors
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Peters P J
Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892.
Hsu V W
Ooi C E
Finazzi D
Teal S B
Oorschot V
Donaldson J G
Klausner R D
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.128.6.1003
Published
1995-03-00
Pages
1003-17
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120412
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.128.6.1003 PMC Full Text View journal details
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