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PMID: 1316805 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

A brefeldin A-like phenotype is induced by the overexpression of a human ERD-2-like protein, ELP-1.

Cell ·Vol. 69 ·No. 4 ·1992-05-15 ·Pages 625-35

Hsu VW, Shah N, Klausner RD

Abstract

Brefeldin A (BFA) is a unique drug affecting the molecular mechanisms that regulate membrane traffic and organelle structure. BFA's ability to alter retrograde traffic from the Golgi to the endoplasmic reticulum (ER) led us to ask whether the ERD-2 retrieval receptor, proposed to return escaped ER resident proteins from the Golgi, might either interfere with or mimic the effects of the drug. When either human ERD-2 or a novel human homolog (referred to as ELP-1) is overexpressed in a variety of cell types, the effects are phenotypically indistinguishable from the addition of BFA. These include the redistribution of the Golgi coat protein, beta-COP, to the cytosol, the loss of the Golgi apparatus as a distinct organelle, the mixing of this organelle with the ER, the addition of complex oligosaccharides to resident ER glycoproteins, and the block of anterograde traffic. Thus, these receptors may provide signals that regulate retrograde traffic between the Golgi and the ER.

MeSH Terms
Amino Acid Sequence Base Sequence Brefeldin A Carrier Proteins Cell Compartmentation/drug effects Cloning, Molecular Cyclopentanes/pharmacology Endocytosis GTP-Binding Proteins/physiology Gene Expression Golgi Apparatus/physiology Humans Membrane Proteins/physiology Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Phenotype Protein Processing, Post-Translational Proteins/metabolism Receptors, Cell Surface/physiology Sequence Alignment Solubility Transfection Vesicular Transport Proteins
Chemicals
Carrier Proteins Cyclopentanes Membrane Proteins Oligodeoxyribonucleotides Proteins Receptors, Cell Surface Vesicular Transport Proteins KDELR2 protein, human Brefeldin A GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hsu V W
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
Shah N
Klausner R D
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(92)90226-3
Published
1992-05-15
Pages
625-35
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC7133352
Subset
IM
Databases
GENBANK
L05499, M87278, M88458, S35159, S72766, S72767, S72768, S72769, S72771, X71664
External Links
PubMed source DOI: 10.1016/0092-8674(92)90226-3 PMC Full Text View journal details
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