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PMID: 2120038 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of a consensus motif for retention of transmembrane proteins in the endoplasmic reticulum.

The EMBO journal ·Vol. 9 ·No. 10 ·1990-10-00 ·Pages 3153-62

Jackson MR, Nilsson T, Peterson PA

Abstract

Several families of transmembrane endoplasmic reticulum (ER) proteins contain retention motifs in their cytoplasmically exposed tails. Mutational analyses demonstrated that two lysines positioned three and four or five residues from the C-terminus represent the retention motif. The introduction of a lysine preceding the lysine that occurs three residues from the terminus of Lyt2 renders this cell surface protein a resident of the ER. Likewise, the appropriate positioning of two lysine residues in a poly-serine sequence confines marker proteins to the ER. Arginines or histidines cannot replace lysines, suggesting that simple charge interactions are not sufficient to explain the retention. The identified consensus motif may serve as a retrieval signal that brings proteins back from a sorting compartment adjacent to the ER.

MeSH Terms
Amino Acid Sequence Animals Antigens, CD/genetics Antigens, Differentiation, T-Lymphocyte/genetics Base Sequence CD8 Antigens DNA, Recombinant/metabolism Endoplasmic Reticulum/metabolism Flow Cytometry Fluorescent Antibody Technique HeLa Cells/metabolism Humans Lysine Membrane Proteins/analysis,genetics Molecular Sequence Data Mutation Oligonucleotide Probes Sequence Homology, Nucleic Acid
Chemicals
Antigens, CD Antigens, Differentiation, T-Lymphocyte CD8 Antigens DNA, Recombinant Membrane Proteins Oligonucleotide Probes Lysine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jackson M R
Department of Immunology, Scripps Clinic and Research Foundation, La Jolla, CA 92037.
Nilsson T
Peterson P A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-10-00
Pages
3153-62
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552044
Subset
IM
Grants
NCI NIH HHS · CA 27489 · United States
External Links
PubMed source PMC Full Text View journal details
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