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PMID: 2333293 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human interleukin 2 receptor beta-chain gene: chromosomal localization and identification of 5' regulatory sequences.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 9 ·1990-05-00 ·Pages 3440-4

Gnarra JR, Otani H, Wang MG, McBride OW, Sharon M, Leonard WJ

Abstract

Interleukin 2 (IL-2) binds to and stimulates activated T cells through high-affinity IL-2 receptors (IL-2Rs). Such receptors represent a complex consisting of at least two proteins, the 55-kDa IL-2R alpha chain and the 70-kDa IL-2R beta chain. The low-affinity, IL-2R alpha chain cannot by itself transduce a mitogenic signal, whereas IL-2 stimulates resting lymphocytes through the intermediate-affinity, IL-2R beta receptor. We report here identification of the genomic locus for IL-2R beta. The exons are contained on four EcoRI fragments of 1.1, 9.2, 7.2, and 13.7 kilobases. The 1.1-kilobase EcoRI fragment lies at the 5'-most end of the genomic locus and contains promoter sequences. The promoter contains no TATA box-like elements but does contain the d(GT)n class of middle repetitive elements, which may play an interesting regulatory role. The IL-2R beta gene is localized to chromosome 22q11.2-q12, a region that is the locus for several lymphoid neoplasias.

MeSH Terms
Base Sequence Blotting, Southern Chromosome Banding Chromosome Mapping Chromosomes, Human, Pair 22 Exons Gene Library Genes Genomic Library Humans Karyotyping Macromolecular Substances Molecular Sequence Data Oligonucleotide Probes Promoter Regions, Genetic Receptors, Interleukin-2/genetics Regulatory Sequences, Nucleic Acid Restriction Mapping
Chemicals
Macromolecular Substances Oligonucleotide Probes Receptors, Interleukin-2
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Gnarra J R
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
Otani H
Wang M G
McBride O W
Sharon M
Leonard W J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.9.3440
Published
1990-05-00
Pages
3440-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53916
Subset
IM
Databases
GENBANK
M32979
External Links
PubMed source DOI: 10.1073/pnas.87.9.3440 PMC Full Text View journal details
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