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PMID: 8335685 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Effects of mutant rat dynamin on endocytosis.

The Journal of cell biology ·Vol. 122 ·No. 3 ·1993-08-00 ·Pages 565-78

Herskovits JS, Burgess CC, Obar RA, Vallee RB

Abstract

Dynamin is a 100-kD microtubule-activated GTPase. Recent evidence has revealed a high degree of sequence homology with the product of the Drosophila gene shibire, mutations in which block the recycling of synaptic vesicles and, more generally, the formation of coated and non-coated vesicles at the plasma membrane. We have now transfected cultured mammalian COS-7 cells with both wild-type and mutant dynamin cDNAs. Point mutations in the GTP-binding consensus sequence elements of dynamin equivalent to dominant negative mutations in ras, and an NH2-terminal deletion of the entire GTP-binding domain of dynamin, block transferrin uptake and alter the distribution of clathrin heavy chain and alpha-, but not gamma-, adaptin. COOH-terminal deletions reverse these effects, identifying this portion of dynamin as a site of interaction with other components of the endocytic pathway. Over-expression of neither wild-type nor mutant forms of dynamin affected the distribution of microtubules. These results demonstrate a specific role for dynamin and for GTP in the initial stages of receptor-mediated endocytosis.

MeSH Terms
Adaptor Protein Complex alpha Subunits Adaptor Proteins, Vesicular Transport Amino Acid Sequence Animals Antibodies/immunology Ca(2+) Mg(2+)-ATPase/chemistry,genetics,immunology,physiology Cell Line Clathrin/analysis,immunology Drosophila Proteins Dynamins Endocytosis Guanosine Triphosphate/metabolism Microscopy, Fluorescence Molecular Sequence Data Mutation Proteins/analysis,immunology Rats Transfection
Chemicals
Adaptor Protein Complex alpha Subunits Adaptor Proteins, Vesicular Transport Antibodies Clathrin Drosophila Proteins Proteins Guanosine Triphosphate Ca(2+) Mg(2+)-ATPase Dynamins shi protein, Drosophila
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Herskovits J S
Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.
Burgess C C
Obar R A
Vallee R B
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.122.3.565
Published
1993-08-00
Pages
565-78
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2119668
Subset
IM
Grants
PHS HHS · 26701 · United States
External Links
PubMed source DOI: 10.1083/jcb.122.3.565 PMC Full Text View journal details
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