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PMID: 8253837 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Hydrolysis of bound GTP by ARF protein triggers uncoating of Golgi-derived COP-coated vesicles.

The Journal of cell biology ·Vol. 123 ·No. 6 Pt 1 ·1993-12-00 ·Pages 1365-71

Tanigawa G, Orci L, Amherdt M, Ravazzola M, Helms JB, Rothman JE

Abstract

The cycle of nucleotide exchange and hydrolysis by a small GTP-binding protein, ADP-ribosylation factor (ARF), helps to provide vectoriality to vesicle transport. Coat assembly is triggered when ARF binds GTP, initiating transport vesicle budding, and coat disassembly is triggered when ARF hydrolyzes GTP, allowing the uncoated vesicle to fuse.

MeSH Terms
ADP-Ribosylation Factors Animals CHO Cells Carrier Proteins/metabolism Cell-Free System Cricetinae GTP-Binding Proteins/metabolism Golgi Apparatus/metabolism,ultrastructure Guanosine Triphosphate/metabolism Membrane Fusion Microscopy, Electron Mutagenesis, Site-Directed Recombinant Proteins Structure-Activity Relationship
Chemicals
Carrier Proteins Recombinant Proteins Guanosine Triphosphate GTP-Binding Proteins ADP-Ribosylation Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Tanigawa G
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York 10021.
Orci L
Amherdt M
Ravazzola M
Helms J B
Rothman J E
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.123.6.1365
Published
1993-12-00
Pages
1365-71
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2290881
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.123.6.1365 PMC Full Text View journal details
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