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PMID: 7896144 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

The quest for a human ori.

Genetica ·Vol. 94 ·No. 2-3 ·1994-00-00 ·Pages 255-66

Falaschi A, Giacca M

Abstract

Attempts at identifying DNA replication origins in human cells have been performed with a variety of molecular genetic and biochemical approaches, with often controversial results. The combination of bromodeoxyuridine labelling, immunopurification of newly synthesized labelled DNA, measurement of the relative abundance of markers in this DNA by quantitative competitive PCR, has allowed the identification within 450 bp of the start-site of DNA replication located at the human lamin B2 gene. The origin is located near the non-transcribed spacer between two highly transcribed genes and shows evidence of a number of specific protein-DNA interactions, the most prominent of which disappears when the cells are differentiated into a non-proliferating state.

MeSH Terms
Chromosome Mapping DNA Replication/genetics Humans Lamin Type B Lamins Models, Genetic Nuclear Proteins/genetics Replication Origin/genetics
Chemicals
Lamin Type B Lamins Nuclear Proteins lamin B2
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Falaschi A
International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
Giacca M
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Article Info
Journal
Genetica
Abbr.
Genetica
ISSN
0016-6707
DOI
10.1007/BF01443439
Published
1994-00-00
Pages
255-66
Language
English
Region
Netherlands
NLM ID
0370740
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01443439 View journal details
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