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PMID: 1291239 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A human DNA replication origin: localization and transcriptional characterization.

Chromosoma ·Vol. 102 ·No. 1 Suppl ·1992-00-00 ·Pages S24-31

Biamonti G, Perini G, Weighardt F, Riva S, Giacca M, Norio P, Zentilin L, Diviacco S, Dimitrova D, Falaschi A

Abstract

A single-copy 13.7 kb human DNA region (L30E) located on Ch. 19 p13.3 contains an origin of DNA replication in myeloid HL-60 cells. The origin was localized, by means of quantitative PCR within approximately 3000 bp, in a highly transcribed region containing at least two closely spaced genes with the same polarity of transcription, one encoding lamin B2 and the other an unidentified protein. The origin region overlaps an undermethylated "CpG island" at the 5'-end of the second transcription unit. A binding site (CACGTG) for basic helix-loop-helix (bHLH) DNA binding proteins such as USF/MLTF or MYC-MAX was located by DNase I footprinting analysis in the promoter of the second gene. DMSO differentiation of HL-60 cells, that completely shuts off replication, also drastically reduces the transcription of L30E region. On the other hand such treatment does not modify the methylation pattern of the CpG island and does not abolish the DNase I protection of the bHLH binding site.

MeSH Terms
Base Sequence Cell Division/genetics Chromosome Mapping Chromosomes, Human, Pair 19 Endodeoxyribonucleases Humans Methylation Molecular Sequence Data Oligodeoxyribonucleotides Promoter Regions, Genetic/genetics Replicon/genetics Transcription, Genetic
Chemicals
Oligodeoxyribonucleotides tri(deoxycytidylic acid-deoxyguanylic acid) Endodeoxyribonucleases deoxyribonuclease II
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Biamonti G
Istituto di Genetica Biochimica ed Evoluzionistica del Consiglio Nazionale delle Ricerche, Pavia, Italy.
Perini G
Weighardt F
Riva S
Giacca M
Norio P
Zentilin L
Diviacco S
Dimitrova D
Falaschi A
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF02451782
Published
1992-00-00
Pages
S24-31
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
Databases
GENBANK
M94363
External Links
PubMed source DOI: 10.1007/BF02451782 View journal details
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