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PMID: 1548776 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Probing protein-DNA interactions at the long terminal repeat of human immunodeficiency virus type 1 by in vivo footprinting.

Journal of virology ·Vol. 66 ·No. 4 ·1992-04-00 ·Pages 2514-8

Demarchi F, D'Agaro P, Falaschi A, Giacca M

Abstract

We have analyzed protein-DNA interactions at the long terminal repeat of human immunodeficiency virus type 1 in a productively infected T-cell line by in vivo dimethyl sulfate footprinting. Major footprints are evident at the basal promoter and enhancer elements. In particular, proteins appear to occupy the TATA box, the Sp1 sites, and the two repeats of the enhancer region. In the negative regulatory element, protections are detected over the USF/MLTF and NFAT-1 sites. Furthermore, two previously unrecognized sites, from nucleotides -260 to -275 and from nucleotides -205 to -216, respectively, appear to be involved in protein-DNA interactions. These two sites are purine rich and share a common sequence motif.

MeSH Terms
Base Sequence Binding Sites Cell Line DNA, Viral/metabolism DNA-Binding Proteins/metabolism HIV Long Terminal Repeat Humans Molecular Sequence Data Sulfuric Acid Esters
Chemicals
DNA, Viral DNA-Binding Proteins Sulfuric Acid Esters dimethyl sulfate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Demarchi F
International Centre for Genetic Engineering and Biotechnology, AREA Science Park, Trieste, Italy.
D'Agaro P
Falaschi A
Giacca M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.4.2514-2518.1992
Published
1992-04-00
Pages
2514-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC289049
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.66.4.2514-2518.1992 PMC Full Text View journal details
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