Home LiteratureArticle Details
PMID: 2302185 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of the human immunodeficiency virus type 1 enhancer-binding proteins from the human T-cell line Jurkat.

The Biochemical journal ·Vol. 265 ·No. 2 ·1990-01-15 ·Pages 547-54

Korner M, Bellan AH, Brini AT, Farrar WL

Abstract

The transcription of the human immunodeficiency virus type 1 (HIV-1) is under the control of cellular proteins that bind to the viral long terminal repeat (LTR). Among the protein-binding regions of the HIV-1 LTR is the transcription-enhancer region. We show that at least one inducible, C1, and one constitutive, C2, protein can bind to the HIV enhancer in Jurkat cells. The two proteins differ in their surface charge, since they are separable by anion-exchange chromatography. Bivalent cations such as Mg2+ and Zn2+ differentially affect their binding to oligonucleotides which contain the HIV-enhancer domain. Both C1 and C2 proteins also bind to a similar sequence found in the interleukin-2-receptor alpha-subunit enhancer. The inducible C1 protein was partially purified by three chromatographic steps and characterized by u.v. cross-linking as a 47 kDa protein.

MeSH Terms
Base Sequence Cell Line Chromatography, Affinity Chromatography, DEAE-Cellulose Chromatography, High Pressure Liquid DNA-Binding Proteins/biosynthesis,isolation & purification,metabolism Enhancer Elements, Genetic HIV-1/genetics,metabolism Humans Molecular Sequence Data Molecular Weight Oligonucleotide Probes T-Lymphocytes Transcription Factors/biosynthesis,isolation & purification,metabolism Transcription, Genetic
Chemicals
DNA-Binding Proteins Oligonucleotide Probes Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Korner M
BCDP, Program Resources, Inc., NCI--Frederick Cancer Research Facility, MD.
Bellan A H
Brini A T
Farrar W L
References (28)
28 references, click to expand
  1. Improved resolution of myofibrillar proteins with sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
    Biochim Biophys Acta. 1977 Jan 25;490(1):27-34 PMID: 836873
  2. NF-kappa B protein purification from bovine spleen: nucleotide stimulation and binding site specificity.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):8825-9 PMID: 2848241
  3. Selective tropism of lymphadenopathy associated virus (LAV) for helper-inducer T lymphocytes.
    Science. 1984 Jul 6;225(4657):59-63 PMID: 6328660
  4. The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus.
    Nature. 1984 Dec 20-1985 Jan 2;312(5996):763-7 PMID: 6096719
  5. Stimulation of in vitro transcription from the SV40 early promoter by the enhancer involves a specific trans-acting factor.
    EMBO J. 1984 Dec 20;3(13):3129-33 PMID: 6098465
  6. The location of cis-acting regulatory sequences in the human T cell lymphotropic virus type III (HTLV-III/LAV) long terminal repeat.
    Cell. 1985 Jul;41(3):813-23 PMID: 2988790
  7. Activation of the AIDS retrovirus promoter by the cellular transcription factor, Sp1.
    Science. 1986 May 9;232(4751):755-9 PMID: 3008338
  8. AIDS retrovirus (ARV-2) clone replicates in transfected human and animal fibroblasts.
    Science. 1986 May 23;232(4753):998-1001 PMID: 3010461
  9. A cyclic AMP- and phorbol ester-inducible DNA element.
    Nature. 1986 Sep 25-Oct 1;323(6086):353-6 PMID: 3020428
  10. Inducibility of kappa immunoglobulin enhancer-binding protein Nf-kappa B by a posttranslational mechanism.
    Cell. 1986 Dec 26;47(6):921-8 PMID: 3096580
  11. Regulation of mRNA accumulation by a human immunodeficiency virus trans-activator protein.
    Cell. 1987 Feb 27;48(4):691-701 PMID: 3643816
  12. Regulation of the human interleukin-2 receptor alpha chain promoter: activation of a nonfunctional promoter by the transactivator gene of HTLV-I.
    Cell. 1987 Apr 10;49(1):47-56 PMID: 3030566
  13. An inducible transcription factor activates expression of human immunodeficiency virus in T cells.
    Nature. 1987 Apr 16-22;326(6114):711-3 PMID: 3031512
  14. Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor.
    Cell. 1987 Jun 19;49(6):729-39 PMID: 3034432
  15. Characterization of cellular proteins recognizing the HIV enhancer using a microscale DNA-affinity precipitation assay.
    Nature. 1987 Nov 26-Dec 2;330(6146):391-5 PMID: 2825023
  16. Purification and characterization of a heat-shock element binding protein from yeast.
    EMBO J. 1987 Oct;6(10):3035-41 PMID: 3319580
  17. DNA affinity labeling of adenovirus type 2 upstream promoter sequence-binding factors identifies two distinct proteins.
    Mol Cell Biol. 1988 Jan;8(1):105-13 PMID: 3336354
  18. Purification of KBF1, a common factor binding to both H-2 and beta 2-microglobulin enhancers.
    EMBO J. 1987 Nov;6(11):3317-24 PMID: 3322806
  19. Interactions of cellular proteins involved in the transcriptional regulation of the human immunodeficiency virus.
    EMBO J. 1987 Dec 1;6(12):3761-70 PMID: 3428273
  20. Two transcription factors, NF-kappa B and H2TF1, interact with a single regulatory sequence in the class I major histocompatibility complex promoter.
    Proc Natl Acad Sci U S A. 1988 Feb;85(3):723-7 PMID: 3422454
  21. The same inducible nuclear proteins regulates mitogen activation of both the interleukin-2 receptor-alpha gene and type 1 HIV.
    Cell. 1988 Jun 3;53(5):827-36 PMID: 2836068
  22. HTLV-1 transactivator induces interleukin-2 receptor expression through an NF-kappa B-like factor.
    Nature. 1988 Jun 23;333(6175):776-8 PMID: 2838755
  23. Identification and purification of a human immunoglobulin-enhancer-binding protein (NF-kappa B) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro.
    Proc Natl Acad Sci U S A. 1988 Jul;85(13):4700-4 PMID: 3133660
  24. Cellular transcription factors and regulation of IL-2 receptor gene expression by HTLV-I tax gene product.
    Science. 1988 Jul 1;241(4861):89-92 PMID: 2838905
  25. Purification of the human immunodeficiency virus type 1 enhancer and TAR binding proteins EBP-1 and UBP-1.
    EMBO J. 1988 Jul;7(7):2117-30 PMID: 3138113
  26. I kappa B: a specific inhibitor of the NF-kappa B transcription factor.
    Science. 1988 Oct 28;242(4878):540-6 PMID: 3140380
  27. Fos and Jun: the AP-1 connection.
    Cell. 1988 Nov 4;55(3):395-7 PMID: 3141060
  28. Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS.
    Science. 1984 May 4;224(4648):500-3 PMID: 6200936
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2650547
Published
1990-01-15
Pages
547-54
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1136918
Subset
IM
Grants
NCI NIH HHS · N01-CO-74102 · United States
External Links
PubMed source DOI: 10.1042/bj2650547 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2