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PMID: 2352322 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel T-cell protein which recognizes a palindromic sequence in the negative regulatory element of the human immunodeficiency virus long terminal repeat.

Journal of virology ·Vol. 64 ·No. 7 ·1990-07-00 ·Pages 3234-9

Orchard K, Perkins N, Chapman C, Harris J, Emery V, Goodwin G, Latchman D, Collins M

Abstract

Two major protein-binding sites within the negative regulatory element of the human immunodeficiency virus type 1 long terminal repeat have been identified. One (site B) contained a palindromic sequence with homology to steroid/thyroid hormone response elements but was distinct from previously described binding sites of this class. A novel T-cell protein recognized the palindromic sequence within site B and also bound estrogen- or thyroid hormone-response elements with lower affinity. A 7-base-pair mutation in the site B palindrome, which destroyed protein binding, resulted in increased expression from the human immunodeficiency virus type 1 long terminal repeat in T cells.

MeSH Terms

Base Sequence Cell Line DNA Mutational Analysis DNA-Binding Proteins/physiology Gene Expression Regulation, Viral HIV-1/genetics Humans In Vitro Techniques Methylation Molecular Sequence Data Nuclear Proteins/physiology Regulatory Sequences, Nucleic Acid Repetitive Sequences, Nucleic Acid Repressor Proteins/physiology T-Lymphocytes/microbiology

Chemicals

DNA-Binding Proteins Nuclear Proteins Repressor Proteins

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Orchard K

Institute of Cancer Research, Chester Beatty Laboratories, London, England.

Perkins N

Chapman C

Harris J

Emery V

Goodwin G

Latchman D

Collins M

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21 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.64.7.3234-3239.1990

Published

1990-07-00

Pages

3234-9

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC249541

Subset

External Links

PubMed source DOI: 10.1128/JVI.64.7.3234-3239.1990 PMC Full Text View journal details

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A novel T-cell protein which recognizes a palindromic sequence in the negative regulatory element of the human immunodeficiency virus long terminal repeat.

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