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PMID: 7896090 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

An implanted recombination hot spot stimulates recombination and enhances sister chromatid cohesion of heterologous YACs during yeast meiosis.

Genetics ·Vol. 138 ·No. 4 ·1994-12-00 ·Pages 1055-65

Sears DD, Hieter P, Simchen G

Abstract

Heterologous yeast artificial chromosomes (YACs) do not recombine with each other and missegregate in 25% of meiosis I events. Recombination hot spots in the yeast Saccharomyces cerevisiae have previously been shown to be associated with sites of meiosis-induced double-strand breaks (DSBs). A 6-kb fragment containing a recombination hot spot/DSB site was implanted onto two heterologous human DNA YACs and was shown to cause the YACs to undergo meiotic recombination in 5-8% of tetrads. Reciprocal exchanges initiated and resolved within the 6-kb insert. Presence of the insert had no detectable effect on meiosis I nondisjunction. Surprisingly, the recombination hot spots acted in cis to significantly reduce precocious sister-chromatid segregation. This novel observation suggests that DSBs are instrumental in maintaining cohesion between sister chromatids in meiosis I. We propose that this previously unknown function of DSBs is mediated by the stimulation of sister-chromatid exchange and/or its intermediates.

MeSH Terms
Base Sequence Chromosomes, Artificial, Yeast/genetics Chromosomes, Fungal DNA, Fungal/genetics DNA, Recombinant/genetics Humans Meiosis Molecular Sequence Data Recombination, Genetic Saccharomyces cerevisiae/genetics Sister Chromatid Exchange
Chemicals
DNA, Fungal DNA, Recombinant
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sears D D
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Hieter P
Simchen G
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/138.4.1055
Published
1994-12-00
Pages
1055-65
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1206247
Subset
IM
Grants
NCI NIH HHS · 5T32CA099139 · United States
NCI NIH HHS · CA16519 · United States
NICHD NIH HHS · HD24605 · United States
External Links
PubMed source DOI: 10.1093/genetics/138.4.1055 PMC Full Text View journal details
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