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PMID: 7885845 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Intra-chromosomal rearrangements generated by Cre-lox site-specific recombination.

Nucleic acids research ·Vol. 23 ·No. 3 ·1995-02-11 ·Pages 485-90

Medberry SL, Dale E, Qin M, Ow DW

Abstract

Chromosomal rearrangements are useful genetic and breeding tools but are often difficult to detect and characterize. To more easily identify and define chromosome deletions and inversions, we have used the bacteriophage P1 Cre-lox site-specific recombination system to generate these events in plants. This involves three steps: (i) the introduction of two lox sites into one locus in a plant genome, including one site within a modified Ds transposon; (ii) Ac transposase-mediated transposition of the Ds-lox element to a new locus on the same chromosome; (iii) Cre-mediated site-specific recombination between the two lox sites that bracket a chromosome segment. We report the production of a deletion and three inversion events in tobacco. The utility of chromosomal segments bracketed by lox sites for targeted manipulation and cloning is discussed.

Related Genes
lox
MeSH Terms
Base Sequence Chromosome Deletion Chromosome Inversion DNA Nucleotidyltransferases DNA Transposable Elements/genetics DNA, Plant/genetics Integrases Molecular Sequence Data Plants, Genetically Modified Plants, Toxic Recombination, Genetic/genetics Tobacco/genetics Viral Proteins
Chemicals
DNA Transposable Elements DNA, Plant Viral Proteins Cre recombinase DNA Nucleotidyltransferases Integrases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Medberry S L
Plant Gene Expression Center, US Department of Agriculture, Albany, CA 94710.
Dale E
Qin M
Ow D W
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/23.3.485
Published
1995-02-11
Pages
485-90
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306701
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/23.3.485 PMC Full Text View journal details
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