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PMID: 1836050 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Gin recombinase of phage Mu can catalyse site-specific recombination in plant protoplasts.

Molecular & general genetics : MGG ·Vol. 230 ·No. 1-2 ·1991-11-00 ·Pages 170-6

Maeser S, Kahmann R

Abstract

A mutant Gin recombinase of the phage Mu DNA inversion system was successfully expressed in Arabidopsis thaliana and tobacco protoplasts. Site-specific recombination was monitored both physically and biologically with the help of a recombination assay system in which expression of a beta-glucuronidase (gus) gene requires Gin-mediated recombination. We demonstrate that the wild-type Gin protein is not able to promote recombination in plant protoplasts, presumably because plant cells do not contain a protein that can substitute for the Escherichia coli FIS protein needed for full activity of wild-type Gin in E. coli. A FIS-independent Gin mutant protein on the other hand was efficient in promoting recombination on recombination substrates introduced transiently and on substrates stably integrated into the plant genome. We discuss the various advantages this system can provide for genetic manipulation of plant cells.

Related Genes
gus
MeSH Terms
Bacteriophage mu/enzymology Base Sequence Catalysis Cloning, Molecular DNA/genetics DNA Nucleotidyltransferases/metabolism Gene Expression Molecular Sequence Data Plants/genetics Plants, Genetically Modified Plants, Toxic Plasmids Protoplasts/metabolism Recombination, Genetic Restriction Mapping Tobacco/genetics Transformation, Genetic
Chemicals
DNA DNA Nucleotidyltransferases Gin recombinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Maeser S
Institut für Genbiologische Forschung Berlin GmbH, FRG.
Kahmann R
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36 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00290665
Published
1991-11-00
Pages
170-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00290665
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