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PMID: 7815528 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

cis-Acting components of human papillomavirus (HPV) DNA replication: linker substitution analysis of the HPV type 11 origin.

Journal of virology ·Vol. 69 ·No. 2 ·1995-02-00 ·Pages 651-60

Russell J, Botchan MR

Abstract

Papillomavirus DNA replication requires the viral trans-acting factors E1 and E2 in addition to the host cell's general replication machinery. The origins of DNA replication in bovine and human papillomavirus genomes have been localized to a specific part of the upstream regulatory region (URR) which includes recognition sites for E1 and E2 proteins. To fine map cis-acting elements influencing human papillomavirus type 11 (HPV-11) DNA replication and to determine the relative contributions of such sites, we engineered consecutive linker substitution mutations across a region of 158 bp in the HPV-11 origin and tested mutant origins for replication function in a cell-based transient replication assay. Our results both confirm and extend the findings of others. E2 binding sites are the major cis components of HPV-11 DNA replication, and there is evidence for synergy between these sites. Differential capacity of the three E2 binding sites within the origin to affect replication may be attributed, at least in part, to context. At least one E2 binding site is essential for replication. The imperfect AT-rich palindrome of the E1 helicase binding site is not essential since replication occurs even in the absence of this sequence. However, replication is enhanced by the presence of the palindromic sequence in the HPV-11 origin. Sequence components adjacent to the E1 and E2 binding sites, comprising AT-rich and purine-rich elements and the consensus TATA box sequence, probably contribute to the overall efficiency of replication, though they are nonessential. None of the other cis elements of the HPV-11 origin region analyzed seems to influence replication significantly in the system described. The HPV-11 origin of DNA replication therefore differs from those of the other papovaviruses, simian virus 40 and polyomavirus, inasmuch as an intact helicase binding site and adjacent AT-rich components, while influential, are not absolutely essential.

MeSH Terms
Animals Base Sequence Binding Sites CHO Cells Cricetinae DNA Replication Genes, Regulator Molecular Sequence Data Papillomaviridae/genetics Trans-Activators/metabolism Virus Replication
Chemicals
Trans-Activators
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Russell J
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Botchan M R
References (86)
86 references, click to expand
  1. Transient replication of human papillomavirus DNAs.
    J Virol. 1992 Oct;66(10):5949-58 PMID: 1326651
  2. Direct interaction between Sp1 and the BPV enhancer E2 protein mediates synergistic activation of transcription.
    Cell. 1991 May 3;65(3):493-505 PMID: 1850324
  3. Cell-free replication of the human papillomavirus DNA with homologous viral E1 and E2 proteins and human cell extracts.
    J Biol Chem. 1994 Sep 30;269(39):24058-65 PMID: 7523366
  4. Origins of DNA replication that function in eukaryotic cells.
    Curr Opin Cell Biol. 1993 Jun;5(3):434-41 PMID: 8352960
  5. Polyomavirus origin for DNA replication comprises multiple genetic elements.
    J Virol. 1983 Sep;47(3):586-99 PMID: 6312083
  6. Trans-plication factors?
    Curr Biol. 1993 Oct 1;3(10):709-12 PMID: 15335868
  7. Duplex opening by dnaA protein at novel sequences in initiation of replication at the origin of the E. coli chromosome.
    Cell. 1988 Mar 11;52(5):743-55 PMID: 2830993
  8. DNA helicase and duplex DNA fragment unwinding activities of polyoma and simian virus 40 large T antigen display similarities and differences.
    J Biol Chem. 1991 Jul 5;266(19):12668-75 PMID: 1648101
  9. Identification of the origin of replication of bovine papillomavirus and characterization of the viral origin recognition factor E1.
    EMBO J. 1991 Dec;10(13):4321-9 PMID: 1661672
  10. Transcription factor E2 regulates BPV-1 DNA replication in vitro by direct protein-protein interaction.
    Cold Spring Harb Symp Quant Biol. 1991;56:335-46 PMID: 1668086
  11. Autonomous DNA replication in human cells is affected by the size and the source of the DNA.
    Mol Cell Biol. 1991 Apr;11(4):2263-72 PMID: 1900922
  12. Replication structure of the human beta-globin gene domain.
    Nature. 1993 Dec 9;366(6455):588-90 PMID: 8255298
  13. Specialized nucleoprotein structures at the origin of replication of bacteriophage lambda: localized unwinding of duplex DNA by a six-protein reaction.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7638-42 PMID: 3020552
  14. Viral E1 and E2 proteins support replication of homologous and heterologous papillomaviral origins.
    Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5799-803 PMID: 1321423
  15. The cellular DNA polymerase alpha-primase is required for papillomavirus DNA replication and associates with the viral E1 helicase.
    Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8700-4 PMID: 8078945
  16. Nuclear factor I enhances adenovirus DNA replication by increasing the stability of a preinitiation complex.
    EMBO J. 1992 Feb;11(2):751-60 PMID: 1537346
  17. Specialized nucleoprotein structures at the origin of replication of bacteriophage lambda: complexes with lambda O protein and with lambda O, lambda P, and Escherichia coli DnaB proteins.
    Proc Natl Acad Sci U S A. 1985 Jul;82(14):4678-82 PMID: 2991888
  18. Specific transcription factors stimulate simian virus 40 and polyomavirus origins of DNA replication.
    Mol Cell Biol. 1992 Jun;12(6):2514-24 PMID: 1317005
  19. Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.
    J Virol. 1990 Feb;64(2):509-18 PMID: 2153220
  20. E2 polypeptides encoded by bovine papillomavirus type 1 form dimers through the common carboxyl-terminal domain: transactivation is mediated by the conserved amino-terminal domain.
    Proc Natl Acad Sci U S A. 1989 Jan;86(2):510-4 PMID: 2536165
  21. The bovine papillomavirus origin of replication requires a binding site for the E2 transcriptional activator.
    Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):898-902 PMID: 8381536
  22. Transactivation of a bovine papilloma virus transcriptional regulatory element by the E2 gene product.
    Cell. 1985 Aug;42(1):183-91 PMID: 2990724
  23. Site-specific DNA-binding proteins important for replication and transcription have multiple activities.
    Cold Spring Harb Symp Quant Biol. 1993;58:403-13 PMID: 7956054
  24. Initiation of simian virus 40 DNA replication in vitro: pulse-chase experiments identify the first labeled species as topologically unwound.
    Proc Natl Acad Sci U S A. 1989 Jun;86(11):3944-8 PMID: 2542958
  25. Simian virus 40 DNA replication in vitro: identification of multiple stages of initiation.
    Mol Cell Biol. 1989 Sep;9(9):3839-49 PMID: 2550804
  26. The acidic transcriptional activation domains of VP16 and p53 bind the cellular replication protein A and stimulate in vitro BPV-1 DNA replication.
    Cell. 1993 Jun 18;73(6):1207-21 PMID: 8390328
  27. Transcription factors and the control of DNA replication.
    Curr Opin Cell Biol. 1992 Jun;4(3):459-67 PMID: 1497917
  28. ATP-dependent formation of a specialized nucleoprotein structure by simian virus 40 (SV40) large tumor antigen at the SV40 replication origin.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):8981-5 PMID: 2827164
  29. Plasmid replication in Xenopus eggs and egg extracts: a 2D gel electrophoretic analysis.
    Nucleic Acids Res. 1992 Apr 11;20(7):1463-9 PMID: 1349740
  30. Bovine papillomavirus E2 trans-activating gene product binds to specific sites in papillomavirus DNA.
    Nature. 1987 Jan 1-7;325(6099):70-3 PMID: 3025749
  31. Bovine papilloma virus plasmids replicate randomly in mouse fibroblasts throughout S phase of the cell cycle.
    Cell. 1987 Jul 3;50(1):59-68 PMID: 3036365
  32. A yeast chromosomal origin of DNA replication defined by multiple functional elements.
    Science. 1992 Feb 14;255(5046):817-23 PMID: 1536007
  33. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92 PMID: 3881765
  34. How transcription factors regulate origins of DNA replication in eukaryotic cells.
    Trends Cell Biol. 1993 May;3(5):161-7 PMID: 14731611
  35. DNA-binding domain of bovine papillomavirus type 1 E1 helicase: structural and functional aspects.
    J Virol. 1993 Oct;67(10):6000-14 PMID: 8396665
  36. Initiation protein induced helix destabilization at the lambda origin: a prepriming step in DNA replication.
    Cell. 1988 Feb 12;52(3):385-95 PMID: 2830983
  37. Intramolecular DNA triplexes, bent DNA and DNA unwinding elements in the initiation region of an amplified dihydrofolate reductase replicon.
    J Mol Biol. 1990 Jan 5;211(1):19-33 PMID: 2299670
  38. Functional organization of the simian virus 40 origin of DNA replication.
    Mol Cell Biol. 1986 Apr;6(4):1117-28 PMID: 3023870
  39. Initiation of simian virus 40 DNA replication in vitro: large-tumor-antigen- and origin-dependent unwinding of the template.
    Proc Natl Acad Sci U S A. 1987 Jun;84(11):3643-7 PMID: 3035543
  40. Specific recognition nucleotides and their DNA context determine the affinity of E2 protein for 17 binding sites in the BPV-1 genome.
    Genes Dev. 1989 Apr;3(4):510-26 PMID: 2542129
  41. Transcriptional activator nuclear factor I stimulates the replication of SV40 minichromosomes in vivo and in vitro.
    Cell. 1989 Nov 3;59(3):541-51 PMID: 2553273
  42. Localized melting and structural changes in the SV40 origin of replication induced by T-antigen.
    EMBO J. 1988 Oct;7(10):3149-58 PMID: 2846276
  43. The E2 binding sites determine the efficiency of replication for the origin of human papillomavirus type 18.
    Nucleic Acids Res. 1992 Nov 25;20(22):6015-21 PMID: 1334259
  44. Acidic transcription factors alleviate nucleosome-mediated repression of DNA replication of bovine papillomavirus type 1.
    Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7051-5 PMID: 8041744
  45. ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex.
    Nature. 1992 May 14;357(6374):128-34 PMID: 1579162
  46. Binding of bovine papillomavirus E1 to the origin is not sufficient for DNA replication.
    Virology. 1993 Mar;193(1):201-12 PMID: 8382395
  47. Characterization of the cis elements involved in basal and E2-transactivated expression of the bovine papillomavirus P2443 promoter.
    J Virol. 1991 Feb;65(2):743-53 PMID: 1846195
  48. The genetics of bovine papillomavirus type 1.
    Annu Rev Genet. 1988;22:235-58 PMID: 2853608
  49. The dnaA protein complex with the E. coli chromosomal replication origin (oriC) and other DNA sites.
    Cell. 1984 Oct;38(3):889-900 PMID: 6091903
  50. Targeting the E1 replication protein to the papillomavirus origin of replication by complex formation with the E2 transactivator.
    Science. 1990 Dec 21;250(4988):1694-9 PMID: 2176744
  51. The T-antigen-binding domain of the simian virus 40 core origin of replication.
    J Virol. 1987 Jul;61(7):2143-9 PMID: 3035215
  52. Two E2 binding sites (E2BS) alone or one E2BS plus an A/T-rich region are minimal requirements for the replication of the human papillomavirus type 11 origin.
    J Virol. 1993 Dec;67(12):7131-9 PMID: 8230435
  53. Differential transcriptional activation by Oct-1 and Oct-2: interdependent activation domains induce Oct-2 phosphorylation.
    Cell. 1990 Feb 9;60(3):375-86 PMID: 2302733
  54. Domain structure of the simian virus 40 core origin of replication.
    Mol Cell Biol. 1986 May;6(5):1663-70 PMID: 3023900
  55. Purification and characterization of replication protein A, a cellular protein required for in vitro replication of simian virus 40 DNA.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2523-7 PMID: 2833742
  56. Control of human papillomavirus type 11 origin of replication by the E2 family of transcription regulatory proteins.
    J Virol. 1992 Sep;66(9):5224-31 PMID: 1323690
  57. A dimer of BPV-1 E2 containing a protease resistant core interacts with its DNA target.
    EMBO J. 1988 Dec 1;7(12):3807-16 PMID: 2850174
  58. Two cellular single-strand-specific DNA-binding proteins interact with two regions of the bovine papillomavirus type 1 genome, including the origin of DNA replication.
    J Virol. 1992 Oct;66(10):5988-98 PMID: 1326653
  59. Characterization of human papillomavirus type 11 E1 and E2 proteins expressed in insect cells.
    J Virol. 1993 May;67(5):2655-63 PMID: 8386271
  60. Activation of BPV-1 replication in vitro by the transcription factor E2.
    Nature. 1991 Oct 17;353(6345):628-32 PMID: 1656277
  61. DNA binding specificity of the bovine papillomavirus E1 protein is determined by sequences contained within an 18-base-pair inverted repeat element at the origin of replication.
    J Virol. 1994 Feb;68(2):1094-102 PMID: 8289339
  62. Binding and unwinding--how T antigen engages the SV40 origin of DNA replication.
    Cell. 1990 Jan 26;60(2):181-4 PMID: 2153460
  63. DNA replication: enzymology and mechanisms.
    Curr Opin Genet Dev. 1994 Apr;4(2):185-95 PMID: 8032195
  64. Bovine papilloma virus (BPV)-encoded E1 protein contains multiple activities required for BPV DNA replication.
    Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):702-6 PMID: 8380645
  65. Formation of the complex of bovine papillomavirus E1 and E2 proteins is modulated by E2 phosphorylation and depends upon sequences within the carboxyl terminus of E1.
    Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6363-7 PMID: 1648739
  66. Polyoma virus DNA replication requires an enhancer.
    Nature. 1984 Nov 15-21;312(5991):242-6 PMID: 6095089
  67. The 68-kilodalton E1 protein of bovine papillomavirus is a DNA binding phosphoprotein which associates with the E2 transcriptional activator in vitro.
    J Virol. 1991 Feb;65(2):649-56 PMID: 1846189
  68. The adenine-thymine domain of the simian virus 40 core origin directs DNA bending and coordinately regulates DNA replication.
    Mol Cell Biol. 1986 Dec;6(12):4578-84 PMID: 3025662
  69. The HeLa Pur factor binds single-stranded DNA at a specific element conserved in gene flanking regions and origins of DNA replication.
    Mol Cell Biol. 1992 Mar;12(3):1257-65 PMID: 1545807
  70. Human papillomavirus type 11 E2 proteins repress the homologous E6 promoter by interfering with the binding of host transcription factors to adjacent elements.
    J Virol. 1994 Feb;68(2):1115-27 PMID: 8289341
  71. Initiation of eukaryotic DNA replication in vitro.
    Annu Rev Cell Biol. 1989;5:197-245 PMID: 2557059
  72. Non-contiguous segments of the polyoma genome required in cis for DNA replication.
    J Mol Biol. 1982 Nov 15;161(4):533-50 PMID: 6296399
  73. Analysis of chromosomal replicons in early embryos of Drosophila melanogaster by two-dimensional gel electrophoresis.
    Nucleic Acids Res. 1991 Jul 25;19(14):3935-41 PMID: 1907366
  74. Elements in the transcriptional regulatory region flanking herpes simplex virus type 1 oriS stimulate origin function.
    J Virol. 1991 May;65(5):2601-11 PMID: 1850034
  75. A bovine papillomavirus E1-related protein binds specifically to bovine papillomavirus DNA.
    J Virol. 1991 Oct;65(10):5314-22 PMID: 1654443
  76. Transcriptional enhancer factor (TEF)-1 and its cell-specific co-activator activate human papillomavirus-16 E6 and E7 oncogene transcription in keratinocytes and cervical carcinoma cells.
    EMBO J. 1992 Jun;11(6):2271-81 PMID: 1318197
  77. The E1 protein of bovine papilloma virus 1 is an ATP-dependent DNA helicase.
    Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5086-90 PMID: 8389467
  78. Replication of plasmid-derived human papillomavirus type 11 DNA in cultured keratinocytes.
    J Virol. 1992 May;66(5):3220-4 PMID: 1313926
  79. EBNA1 can link the enhancer element to the initiator element of the Epstein-Barr virus plasmid origin of DNA replication.
    J Virol. 1992 Jan;66(1):489-95 PMID: 1309258
  80. Transient replication of BPV-1 requires two viral polypeptides encoded by the E1 and E2 open reading frames.
    EMBO J. 1991 Feb;10(2):449-57 PMID: 1846806
  81. DNA replication initiates at multiple sites on plasmid DNA in Xenopus egg extracts.
    Nucleic Acids Res. 1992 Apr 11;20(7):1457-62 PMID: 1579437
  82. A vector that replicates as a plasmid and can be efficiently selected in B-lymphoblasts transformed by Epstein-Barr virus.
    Mol Cell Biol. 1985 Feb;5(2):410-3 PMID: 2983194
  83. Replication of human papillomavirus (HPV) DNAs supported by the HPV type 18 E1 and E2 proteins.
    J Virol. 1994 Jan;68(1):505-9 PMID: 8254762
  84. Animal virus DNA replication.
    Annu Rev Biochem. 1989;58:671-717 PMID: 2549858
  85. The carboxy-terminal domain shared by the bovine papillomavirus E2 transactivator and repressor proteins contains a specific DNA binding activity.
    EMBO J. 1988 Feb;7(2):533-9 PMID: 2835232
  86. Bovine papilloma virus (BPV)-encoded E2 protein enhances binding of E1 protein to the BPV replication origin.
    Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2865-9 PMID: 8385347
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.69.2.651-660.1995
Published
1995-02-00
Pages
651-60
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC188625
Subset
IM
Grants
NCI NIH HHS · CA-30490 · United States
NCI NIH HHS · CA-42414 · United States
NIEHS NIH HHS · ESO1896 · United States
External Links
PubMed source DOI: 10.1128/JVI.69.2.651-660.1995 PMC Full Text View journal details
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