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PMID: 1656277 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Activation of BPV-1 replication in vitro by the transcription factor E2.

Nature ·Vol. 353 ·No. 6345 ·1991-10-17 ·Pages 628-32

Yang L, Li R, Mohr IJ, Clark R, Botchan MR

Abstract

Soluble extracts from uninfected murine cells supplemented with purified viral E1 and E2 proteins support the replication of exogenously added papilloma virus DNA. The E2 transactivator stimulates the binding of the E1 replication protein to the minimal origin of replication and activates DNA replication. These results support the concept that transcription factors have a direct role in the initiation of DNA replication in eukaryotes by participating in the assembly of a complex at the origin of replication.

MeSH Terms

Aphidicolin/pharmacology Base Sequence Binding Sites Bovine papillomavirus 1/physiology DNA Replication/drug effects DNA, Viral/biosynthesis,genetics,metabolism Kinetics Molecular Sequence Data Regulatory Sequences, Nucleic Acid Templates, Genetic Transcription Factors/pharmacology Virus Replication/drug effects

Chemicals

DNA, Viral Transcription Factors Aphidicolin

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Yang L

Department of Molecular and Cell Biology, University of California, Berkeley 94720.

Li R

Mohr I J

Clark R

Botchan M R

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/353628a0

Published

1991-10-17

Pages

628-32

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/353628a0 View journal details

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Activation of BPV-1 replication in vitro by the transcription factor E2.

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