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PMID: 1323690 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Control of human papillomavirus type 11 origin of replication by the E2 family of transcription regulatory proteins.

Journal of virology ·Vol. 66 ·No. 9 ·1992-09-00 ·Pages 5224-31

Chiang CM, Dong G, Broker TR, Chow LT

Abstract

Replication of human papillomavirus type 11 (HPV-11) DNA requires the full-length viral E1 and E2 proteins (C.-M. Chiang, M. Ustav, A. Stenlund, T. F. Ho, T. R. Broker, and L. T. Chow, Proc. Natl. Acad. Sci. USA 89:5799-5803, 1992). Using transient transfection of subgenomic HPV DNA into hamster CHO and human 293 cells, we have localized an origin of replication (ori) to an 80-bp segment in the upstream regulatory region spanning nucleotide 1. It overlaps the E6 promoter region and contains a short A + T-rich segment and a sequence which is homologous to the binding site of the bovine papillomavirus type 1 (BPV-1) E1 protein in the BPV-1 ori. However, unlike the BPV-1 ori, for which half an E2-responsive sequence (E2-RS) or binding site suffices, an intact binding site is essential for the HPV-11 ori. Replication was more efficient when additional E2-RSs were present. The intact HPV-11 genome also replicated in both cell lines when supplied with E1 and E2 proteins. Expression vectors of transcription repressor proteins that lack the N-terminal domain essential for E2 transcriptional trans activation did not support replication in collaboration with the E1 expression vector. Rather, cotransfection with the repressor expression vectors inhibited ori replication by the E1 and E2 proteins. These results demonstrate the importance of the N-terminal domain of the E2 protein in DNA replication and indicate that the family of E2 proteins positively and negatively regulates both viral DNA replication and E6 promoter transcription.

MeSH Terms
Animals Base Sequence Bovine papillomavirus 1/genetics,physiology CHO Cells Cottontail rabbit papillomavirus/genetics,physiology Cricetinae DNA Mutational Analysis DNA-Binding Proteins/metabolism Genome, Viral Humans Molecular Sequence Data Papillomaviridae/genetics,physiology Rabbits Regulatory Sequences, Nucleic Acid/genetics Repressor Proteins/metabolism Transcription, Genetic Viral Proteins/metabolism Virus Replication
Chemicals
DNA-Binding Proteins E2 protein, Bovine papillomavirus Repressor Proteins Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chiang C M
Department of Biochemistry, School of Medicine and Dentistry, University of Rochester, New York 14642.
Dong G
Broker T R
Chow L T
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.9.5224-5231.1992
Published
1992-09-00
Pages
5224-31
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC289075
Subset
IM
Grants
NCI NIH HHS · CA36200 · United States
External Links
PubMed source DOI: 10.1128/JVI.66.9.5224-5231.1992 PMC Full Text View journal details
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