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PMID: 7808411 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Modification of the 5' untranslated leader region of the maize Activator element leads to increased activity in Arabidopsis.

Molecular & general genetics : MGG ·Vol. 245 ·No. 5 ·1994-12-01 ·Pages 608-15

Lawson EJ, Scofield SR, Sjodin C, Jones JD, Dean C

Abstract

In contrast to its behavior in tobacco and tomato, the maize transposable element Ac is relatively inactive in Arabidopsis. We show here that removal of 537 bp within a CpG-rich region of the Ac 5' untranslated leader region significantly increases the excision frequency of the element in Arabidopsis. This increase did not appear to be correlated with the removal of sequences that are methylated in inactive Ac elements in maize, as these sites were not methylated in Ac elements in Arabidopsis transformants. The deletion within the 5' untranslated leader did not increase Ac activity by increasing levels of steady-state transposase mRNA, as assayed by RNase protection experiments. Moreover, there was no correlation between the levels of steady-state transposase mRNA and Ac element activity. This suggests that post-transcriptional regulation of Ac activity occurs in Arabidopsis.

MeSH Terms

Arabidopsis/genetics DNA Transposable Elements/genetics Drug Resistance/genetics Genetic Markers Glucuronidase/genetics Kanamycin Resistance/genetics Methylation Nucleotidyltransferases/genetics RNA, Messenger/genetics RNA, Plant/genetics Sequence Deletion Streptomycin/pharmacology Transformation, Genetic Transposases Zea mays/enzymology,genetics

Chemicals

DNA Transposable Elements Genetic Markers RNA, Messenger RNA, Plant Nucleotidyltransferases Transposases Glucuronidase Streptomycin

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Lawson E J

Institute of Plant Science Research (ISPR), John Innes Centre and Sainsbury Laboratory, Norwich, UK.

Scofield S R

Sjodin C

Jones J D

Dean C

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00282223

Published

1994-12-01

Pages

608-15

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00282223

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Modification of the 5' untranslated leader region of the maize Activator element leads to increased activity in Arabidopsis.

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