Behaviour of modified Ac elements in flax callus and regenerated plants.

Plant molecular biology ·Vol. 22 ·No. 4 ·1993-07-00 ·Pages 625-33

Finnegan EJ, Lawrence GJ, Dennis ES, Ellis JG

Abstract

The Ac element of maize has been modified by deletion of 537 bases (delta NaeAc) from the untranslated leader of the transposase gene. In a second modification the cauliflower mosaic virus 35S promoter has been inserted into the truncated leader of delta NaeAc, 21 bases upstream of the natural translation start. The activity of these modified elements has been compared with that of the unmodified element in transgenic flax. Deletion of sequences in the untranslated leader only marginally increased transposition in callus while insertion of the 35S promoter enhanced transposition frequency 7-8-fold. Increased transposition correlated with increased transcription of the transposase gene. The presence of a 35S promoter upstream of the transposase gene, but outside the Ac element, also enhanced both transcription and transposition.

MeSH Terms

Agrobacterium tumefaciens/genetics Base Sequence Cloning, Molecular Culture Techniques DNA Transposable Elements/genetics Glucuronidase/genetics Molecular Sequence Data Nucleotidyltransferases/genetics Plant Development Plants/genetics Plants, Genetically Modified/genetics Promoter Regions, Genetic/genetics Regeneration Sequence Deletion Transformation, Genetic Transposases Zea mays/genetics

Chemicals

DNA Transposable Elements Nucleotidyltransferases Transposases Glucuronidase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Finnegan E J

CSIRO, Division of Plant Industry, Canberra, ACT, Australia.

Lawrence G J

Dennis E S

Ellis J G

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25 references, click to expand

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Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

DOI

10.1007/BF00047403

Published

1993-07-00

Pages

625-33

Language

English

Region

Netherlands

NLM ID

9106343

Subset

External Links

PubMed source DOI: 10.1007/BF00047403 View journal details

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Behaviour of modified Ac elements in flax callus and regenerated plants.

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