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PMID: 1338696 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants.

Transgenic research ·Vol. 1 ·No. 6 ·1992-11-00 ·Pages 285-97

Jones JD, Shlumukov L, Carland F, English J, Scofield SR, Bishop GJ, Harrison K

Abstract

Progress in plant molecular biology has depended heavily on the availability of effective vectors for plant cell transformation and heterologous expression. In this paper we describe the structures of a wide array of plasmids which have proved extremely effective in (a) plant transformation, (b) expression of heterologous genes and (c) assaying the activity of transposons in transgenic plants. Constructs that confer resistance to kanamycin, hygromycin, streptomycin, spectinomycin and phosphinotricin, or that confer beta-glucuronidase (GUS) gene expression are presented. Binary vector constructs that carry polylinkers of the pUC and Bluescript types are also described. Plasmids that permit the expression of any heterologous reading frame from either nopaline synthase (nos) or octopine synthase (ocs) promoters, as well as the cauliflower mosaic virus 35S promoter, using either the nopaline synthase or octopine synthase 3' polyadenylation sequences, are presented. These constructs permit a choice of orientation of the resulting transgene of interest, relative to the orientation of the selection marker gene. Most of the plasmids described here are publicly available.

Related Genes
nos ocs
MeSH Terms
Amino Acid Oxidoreductases/genetics Base Sequence Cloning, Molecular/methods DNA Transposable Elements Drug Resistance/genetics Escherichia coli/genetics Genetic Techniques Genetic Vectors Glucuronidase/genetics,metabolism Molecular Sequence Data Oligodeoxyribonucleotides Plants/genetics Plants, Genetically Modified Plasmids Promoter Regions, Genetic Protein Sorting Signals/genetics Restriction Mapping Transformation, Genetic
Chemicals
DNA Transposable Elements Oligodeoxyribonucleotides Protein Sorting Signals Amino Acid Oxidoreductases nopaline synthase D-octopine dehydrogenase Glucuronidase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Jones J D
John Innes Centre for Plant Science Research, Norwich Research Park, Colney, UK.
Shlumukov L
Carland F
English J
Scofield S R
Bishop G J
Harrison K
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36 references, click to expand
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Article Info
Journal
Transgenic research
Abbr.
Transgenic Res
ISSN
0962-8819
DOI
10.1007/BF02525170
Published
1992-11-00
Pages
285-97
Language
English
Region
Netherlands
NLM ID
9209120
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02525170 View journal details
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