Engineering herbicide resistance in plants by expression of a detoxifying enzyme.

The EMBO journal ·Vol. 6 ·No. 9 ·1987-09-00 ·Pages 2513-8

Block MD, Botterman J, Vandewiele M, Dockx J, Thoen C, Gosselé V, Movva NR, Thompson C, Montagu MV, Leemans J

Abstract

Phosphinothricin (PPT) is a potent inhibitor of glutamine synthetase in plants and is used as a non-selective herbicide. The bar gene which confers resistance in Streptomyces hygroscopicus to bialaphos, a tripeptide containing PPT, encodes a phosphinothricin acetyltransferase (PAT) (see accompanying paper). The bar gene was placed under control of the 35S promoter of the cauliflower mosaic virus and transferred to plant cells using Agrobacterium-mediated transformation. PAT was used as a selectable marker in protoplast co-cultivation. The chimeric bar gene was expressed in tobacco, potato and tomato plants. Transgenic plants showed complete resistance towards high doses of the commercial formulations of phosphinothricin and bialaphos. These data present a successful approach to obtain herbicide-resistant plants by detoxification of the herbicide.

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Block M D

Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium.

Botterman J

Vandewiele M

Dockx J

Thoen C

Gosselé V

Movva N R

Thompson C

Montagu M V

Leemans J

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1987-09-00

Pages

2513-8

Language

English

Region

England

NLM ID

8208664

PMCID

PMC553667

External Links

PubMed source PMC Full Text View journal details

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Engineering herbicide resistance in plants by expression of a detoxifying enzyme.

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