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PMID: 1321434 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

High rates of Ac/Ds germinal transposition in Arabidopsis suitable for gene isolation by insertional mutagenesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 13 ·1992-07-01 ·Pages 6085-9

Grevelding C, Becker D, Kunze R, von Menges A, Fantes V, Schell J, Masterson R

Abstract

Overexpression of the Activator (Ac) transposase gene in Arabidopsis thaliana resulted in a minimal germinal transposition frequency of 27% in which independent Dissociation (Ds) transposition events were observed. Molecular analysis of 45 F1 generation Ac/Ds plants indicated that high rates of somatic excision had occurred, and independent germinal insertions were identified in F2 generation progeny plants. A tandem cauliflower mosaic virus (CaMV) promoter fused to two different Ac coding sequences significantly increased the rate of Ds transposition. The CaMV-Ac fusions activated single and multiple copies of two different Ds elements, DsDHFR and Ds35S-1, and reciprocal crosses resulted in similar transposition frequencies. The improved rate of independent germinal transposition observed makes Arabidopsis an ideal system for insertional mutagenesis.

Related Genes
Ac Ds
MeSH Terms
Base Sequence Blotting, Southern Cloning, Molecular DNA Transposable Elements Gene Expression Genetic Vectors Molecular Sequence Data Mutagenesis, Insertional Nucleotidyltransferases/metabolism Oligodeoxyribonucleotides/chemistry Plants/genetics RNA, Messenger/genetics Rhizobium/genetics Transposases Zea mays/genetics
Chemicals
DNA Transposable Elements Oligodeoxyribonucleotides RNA, Messenger Nucleotidyltransferases Transposases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Grevelding C
Max-Planck-Institut für Züchtungsforschung, Cologne, Federal Republic of Germany.
Becker D
Kunze R
von Menges A
Fantes V
Schell J
Masterson R
References (28)
28 references, click to expand
  1. Excision of a Ds-like maize transposable element (Ac delta) in a transient assay in Petunia is enhanced by a truncated coding region of the transposable element Ac.
    Mol Gen Genet. 1990 Oct;224(1):17-23 PMID: 2177522
  2. Transcription of transposable element Activator (Ac) of Zea mays L.
    EMBO J. 1987 Jun;6(6):1555-63 PMID: 16453772
  3. Duplication of CaMV 35S Promoter Sequences Creates a Strong Enhancer for Plant Genes.
    Science. 1987 Jun 5;236(4806):1299-302 PMID: 17770331
  4. Mutational analysis of the N terminus of the protein of maize transposable element Ac.
    Proc Natl Acad Sci U S A. 1990 Aug;87(16):6044-8 PMID: 2166942
  5. Construction and characterization of a yeast artificial chromosome library of Arabidopsis which is suitable for chromosome walking.
    Mol Gen Genet. 1991 May;226(3):484-90 PMID: 1674816
  6. Isolation of single-copy-sequence clones from a yeast artificial chromosome library of randomly-sheared Arabidopsis thaliana DNA.
    Plant Mol Biol. 1990 Apr;14(4):561-8 PMID: 2102834
  7. A comparison of eukaryotic viral 5'-leader sequences as enhancers of mRNA expression in vivo.
    Nucleic Acids Res. 1987 Nov 11;15(21):8693-711 PMID: 2825117
  8. Restriction Fragment Length Polymorphism Linkage Map of Arabidopsis thaliana.
    Plant Cell. 1989 Jul;1(7):699-705 PMID: 12359906
  9. Sexual transmission of transposed activator elements in transgenic tomatoes.
    Genetics. 1989 Sep;123(1):181-9 PMID: 17246510
  10. Transposable elements can be used to study cell lineages in transgenic plants.
    Plant Cell. 1989 Aug;1(8):757-64 PMID: 2562511
  11. Phenotypic assay for excision of the maize controlling element Ac in tobacco.
    EMBO J. 1987 Jun;6(6):1547-54 PMID: 16453771
  12. Transposition of the maize controlling element "Activator" in tobacco.
    Proc Natl Acad Sci U S A. 1986 Jul;83(13):4844-8 PMID: 16593722
  13. Properties of the maize transposable element Activator in transgenic tobacco plants: a versatile inter-species genetic tool.
    Plant Cell. 1990 Aug;2(8):709-21 PMID: 1967055
  14. The maize transposable element Ac excises in progeny of transformed tobacco.
    Plant Mol Biol. 1989 Jul;13(1):109-18 PMID: 2562778
  15. High-frequency T-DNA-mediated gene tagging in plants.
    Proc Natl Acad Sci U S A. 1989 Nov;86(21):8467-71 PMID: 2554318
  16. Identification and map position of YAC clones comprising one-third of the Arabidopsis genome.
    Plant J. 1991 Nov;1(3):367-74 PMID: 1844889
  17. The use of transgenic plants to understand transposition mechanisms and to develop transposon tagging strategies.
    Plant Mol Biol. 1991 Mar;16(3):449-61 PMID: 1654159
  18. Chromosome organization and genic expression.
    Cold Spring Harb Symp Quant Biol. 1951;16:13-47 PMID: 14942727
  19. Preferential transposition of the maize element Activator to linked chromosomal locations in tobacco.
    Plant Cell. 1990 Aug;2(8):701-7 PMID: 1967054
  20. Sequences near the termini are required for transposition of the maize transposon Ac in transgenic tobacco plants.
    Proc Natl Acad Sci U S A. 1989 Dec;86(23):9385-8 PMID: 2556710
  21. A Dwarf Mutant of Arabidopsis Generated by T-DNA Insertion Mutagenesis.
    Science. 1989 Mar 10;243(4896):1351-4 PMID: 17808268
  22. Induced transposition of Ds by a stable Ac in crosses of transgenic tobacco plants.
    Mol Gen Genet. 1989 May;217(1):53-9 PMID: 2549368
  23. Restriction fragment length polymorphism linkage map for Arabidopsis thaliana.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6856-60 PMID: 2901107
  24. Purification of mRNA directly from crude plant tissues in 15 minutes using magnetic oligo dT microspheres.
    Nucleic Acids Res. 1990 Jun 25;18(12):3669 PMID: 2362831
  25. Visual detection of transposition of the maize element activator (ac) in tobacco seedlings.
    Science. 1989 Apr 14;244(4901):204-7 PMID: 17835353
  26. Studies on the introduction and mobility of the maize Activator element in Arabidopsis thaliana and Daucus carota.
    EMBO J. 1987 Dec 20;6(13):3881-9 PMID: 2832144
  27. Binary vectors which allow the exchange of plant selectable markers and reporter genes.
    Nucleic Acids Res. 1990 Jan 11;18(1):203 PMID: 2408008
  28. Characterization of the maize transposable element Ac by internal deletions.
    EMBO J. 1988 Dec 1;7(12):3653-9 PMID: 16453860
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.13.6085
Published
1992-07-01
Pages
6085-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49442
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.13.6085 PMC Full Text View journal details
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