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PMID: 7739559 Published · ppublish English Journal Article

Upstream stimulatory factor regulates expression of the cell cycle-dependent cyclin B1 gene promoter.

Molecular and cellular biology ·Vol. 15 ·No. 5 ·1995-05-00 ·Pages 2782-90

Cogswell JP, Godlevski MM, Bonham M, Bisi J, Babiss L

Abstract

Progression through the somatic cell cycle requires the temporal regulation of cyclin gene expression and cyclin protein turnover. One of the best-characterized examples of this regulation is seen for the B-type cyclins. These cyclins and their catalytic component, cdc2, have been shown to mediate both the entry into and maintenance of mitosis. The cyclin B1 gene has been shown to be expressed between the late S and G2 phases of the cell cycle, while the protein is degraded specifically at interphase via ubiquitination. To understand the molecular basis for transcriptional regulation of the cyclin B1 gene, we cloned the human cyclin B1 gene promoter region. Using a chloramphenicol acetyltransferase reporter system and both stable and transient assays, we have shown that the cyclin B1 gene promoter (extending to -3800 bp relative to the cap site) can confer G2-enhanced promoter activity. Further analysis revealed that an upstream stimulatory factor (USF)-binding site and its cognate transcription factor(s) are critical for expression from the cyclin B1 promoter in cycling HeLa cells. Interestingly, USF DNA-binding activity appears to be regulated in a G2-specific fashion, supporting the idea that USF may play some role in cyclin B1 gene activation. These studies suggest an important link between USF and the cyclin B1 gene, which in part explains how maturation promoting factor complex formation is regulated.

MeSH Terms
Amino Acid Sequence Base Sequence Binding Sites/genetics Cell Cycle/genetics Cyclins/genetics DNA Primers/genetics DNA, Complementary/genetics DNA-Binding Proteins Gene Expression Regulation Genes, Reporter HeLa Cells Humans Molecular Sequence Data Promoter Regions, Genetic Transcription Factors/genetics Transcription, Genetic Transcriptional Activation Upstream Stimulatory Factors
Chemicals
Cyclins DNA Primers DNA, Complementary DNA-Binding Proteins Transcription Factors USF1 protein, human Upstream Stimulatory Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cogswell J P
Department of Molecular Genetics, Glaxo Research Institute, Research Triangle Park, North Carolina 27709, USA.
Godlevski M M
Bonham M
Bisi J
Babiss L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.5.2782
Published
1995-05-00
Pages
2782-90
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230509
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.15.5.2782 PMC Full Text View journal details
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