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PMID: 8313904 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of a novel vertebrate cyclin: cyclin B3 shares properties with both A- and B-type cyclins.

The EMBO journal ·Vol. 13 ·No. 3 ·1994-02-01 ·Pages 595-605

Gallant P, Nigg EA

Abstract

Cyclins play a key role in controlling progression through the cell cycle. They act as regulatory subunits of p34cdc2/CDC28 and related cyclin-dependent protein kinases (cdks). In vertebrates, cyclins B1 and B2 function during M phase, whereas cyclin A is required for S phase as well as the G2 to M phase transition. Here, we describe the identification and characterization of a novel vertebrate cyclin, termed cyclin B3. The assignment of this cyclin to the B-type subfamily is based on its cDNA-derived sequence and its pattern of expression in synchronized cells, both suggesting a distant relationship to other B-type cyclins. Interestingly, however, cyclin B3 also displays properties that resemble those of A- rather than B-type cyclins. Specifically, cyclin B3 localizes to the cell nucleus throughout the cell cycle, and is able to associate in vivo with at least two kinase subunits, p34cdc2 and p33cdk2. Furthermore, deletion of 26 amino acids from the C-terminus of cyclin B3 impairs both its interaction with kinase catalytic subunits and its nuclear localization, reminiscent of recent results obtained with cyclin A. Based on these observations, we conclude that cyclin B3 may share functional properties with both A- and B-type cyclins.

MeSH Terms
Amino Acid Sequence Animals Base Sequence CDC2 Protein Kinase/metabolism CDC2-CDC28 Kinases Cell Line Chick Embryo Cloning, Molecular Cyclin B Cyclin-Dependent Kinase 2 Cyclin-Dependent Kinases Cyclins/analysis,genetics DNA HeLa Cells Humans Immunohistochemistry Molecular Sequence Data Protein Kinases/metabolism Protein Serine-Threonine Kinases Sequence Homology, Amino Acid Vertebrates
Chemicals
CCNB3 protein, human Cyclin B Cyclins DNA Protein Kinases Protein Serine-Threonine Kinases CDC2 Protein Kinase CDC2-CDC28 Kinases CDK2 protein, human Cyclin-Dependent Kinase 2 Cyclin-Dependent Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gallant P
Swiss Institute for Experimental Cancer Research (ISREC), Epalinges.
Nigg E A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-02-01
Pages
595-605
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394849
Subset
IM
Databases
GENBANK
X75757
External Links
PubMed source PMC Full Text View journal details
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