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PMID: 1824935 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cyclin B in Xenopus oocytes: implications for the mechanism of pre-MPF activation.

The EMBO journal ·Vol. 10 ·No. 1 ·1991-01-00 ·Pages 177-82

Gautier J, Maller JL

Abstract

Using a polyclonal antibody raised against B2 cyclin from Xenopus laevis, we show that prophase-arrested Xenopus oocytes contain a stockpile of cyclin B2 protein. During progesterone-induced maturation, an increase in the synthesis of cyclin B2 is observed, although Western blotting experiments show that this new synthesis does not significantly increase the mass of cyclin over the maternal stockpile. In the oocyte cyclin B2 is already present in two forms which differ in the extent of phosphorylation, but the phosphorylated form becomes predominant as oocytes progress towards germinal vesicle breakdown (GVBD), coincident with cdc2 protein kinase activation. These two events do not depend upon formation of a new complex between cyclin and cdc2 protein kinase, since these two proteins are already found associated in resting oocytes, prior to activation of the kinase.

MeSH Terms
Animals Antibodies Blotting, Western CDC2 Protein Kinase/metabolism Cyclins/isolation & purification,metabolism Enzyme Activation Female Kinetics Maturation-Promoting Factor/metabolism Oocytes/physiology Phosphates/metabolism Phosphorylation Protein Precursors/metabolism Protein Processing, Post-Translational Xenopus laevis
Chemicals
Antibodies Cyclins Phosphates Protein Precursors maturation-promoting factor precursor CDC2 Protein Kinase Maturation-Promoting Factor
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gautier J
Department of Pharmacology and Howard Hughes Medical Institute, University of Colorado School of Medicine, Denver 80262.
Maller J L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-01-00
Pages
177-82
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452627
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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