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PMID: 1549117 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Evidence that USF can interact with only a single general transcription complex at one time.

Molecular and cellular biology ·Vol. 12 ·No. 4 ·1992-04-00 ·Pages 1630-8

Adami G, Babiss LE

Abstract

By in vitro analysis, the major late promoter (MLP) of adenovirus has been shown to be a simple promoter requiring two elements for efficient transcription: a minimal promoter element (MPE), where the general transcription factor-polymerase II complex binds, and a single functional upstream promoter element (UPE) which interacts with USF. Two hundred bases upstream of the MLP cap site and divergently oriented is the IVa2 promoter. This promoter has its own MPE but shares the MLP UPE, suggesting the possibility that these promoters are coordinately regulated. To determine mechanistically how this might function, we replaced the weak IVa2 minimal promoter with a strong MPE (from the viral E1A gene) and observed mutual inhibition of both promoters and unstable transcription factor binding. Only by duplication of the UPE could this inhibition be relieved. When tested independently, both promoters were shown to require the USF site for maximal activity. These results are compatible with a model in which USF can stably interact with only one transcription complex at a time. When two divergently oriented general transcription complexes compete efficiently for binding of USF, transcription is reduced to the same levels as if the USF site were absent.

MeSH Terms
Adenoviridae/genetics Base Sequence Binding, Competitive DNA, Recombinant/genetics DNA-Binding Proteins Gene Expression Regulation, Viral Molecular Sequence Data Promoter Regions, Genetic/genetics TATA Box/genetics Transcription Factors/metabolism Transcription, Genetic Upstream Stimulatory Factors
Chemicals
DNA, Recombinant DNA-Binding Proteins Transcription Factors Upstream Stimulatory Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Adami G
Laboratory of Molecular Cell Biology, Rockefeller University, New York, New York 10021.
Babiss L E
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.4.1630-1638.1992
Published
1992-04-00
Pages
1630-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369606
Subset
IM
Grants
NCI NIH HHS · CA48707 · United States
External Links
PubMed source DOI: 10.1128/mcb.12.4.1630-1638.1992 PMC Full Text View journal details
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