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PMID: 1903838 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

cis-acting sequences required for expression of the divergently transcribed Drosophila melanogaster Sgs-7 and Sgs-8 glue protein genes.

Molecular and cellular biology ·Vol. 11 ·No. 6 ·1991-06-00 ·Pages 2971-9

Hofmann A, Garfinkel MD, Meyerowitz EM

Abstract

The Sgs-7 and Sgs-8 glue genes at 68C are divergently transcribed and are separated by 475 bp. Fusion genes with Adh or lacZ coding sequences were constructed, and the expression of these genes, with different amounts of upstream sequences present, was tested by a transient expression procedure and by germ line transformation. A cis-acting element for both genes is located asymmetrically in the intergenic region between -211 and -43 bp relative to Sgs-7. It is required for correct expression of both genes. This element can confer the stage- and tissue-specific expression pattern of glue genes on a heterologous promoter. An 86-bp portion of the element, from -133 to -48 bp relative to Sgs-7, is shown to be capable of enhancing the expression of a truncated and therefore weakly expressed Sgs-3 fusion gene. Recently described common sequence motifs of glue gene regulatory elements (T. Todo, M. Roark, K. Vijay Raghavan, C. A. Mayeda, and E.M. Meyerowitz, Mol. Cell. Biol. 10:5991-6002, 1990) are located within this 86-bp region.

Related Genes
Sgs-7 Sgs-8
MeSH Terms
Animals Base Sequence Cloning, Molecular DNA, Ribosomal/genetics Drosophila melanogaster/genetics Escherichia coli/genetics Gene Expression Genes Glue Proteins, Drosophila/genetics Molecular Sequence Data Plasmids Regulatory Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Transcription, Genetic Transformation, Genetic
Chemicals
DNA, Ribosomal Glue Proteins, Drosophila
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hofmann A
Division of Biology, California Institute of Technology, Pasadena 91125.
Garfinkel M D
Meyerowitz E M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.6.2971-2979.1991
Published
1991-06-00
Pages
2971-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360127
Subset
IM
Grants
NIGMS NIH HHS · 5 T32 GM07616 · United States
NIGMS NIH HHS · GM28075 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.6.2971-2979.1991 PMC Full Text View journal details
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