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PMID: 3128796 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of a DNase I-hypersensitive site in transgenic Drosophila reveals a key regulatory element of Sgs3.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 8 ·1988-04-00 ·Pages 2718-22

Ramain P, Giangrande A, Richards G, Bellard M

Abstract

We have undertaken chromatin studies on transformed Drosophila strains carrying DNA sequences modified in the region of the DNase I (EC 3.1.4.5)-hypersensitive sites -750 and -600 base pairs upstream from the Sgs3 start site. Although both sites are developmentally specific, modifications in the -750 site have little or no effect on Sgs3-encoded transcript levels, whereas either deletion or replacement of sequences at the -600 site causes an important reduction in transcript levels. The element associated with the -600 site enhances Sgs3 transcription when displaced with respect to the start site. This combined approach has defined sequence elements necessary both for normal transcript levels as well as the chromatin structure characteristic of Sgs3 activity in vivo.

MeSH Terms

Animals Animals, Genetically Modified/genetics Base Sequence Chromatin/ultrastructure DNA Mutational Analysis Deoxyribonuclease I/metabolism Drosophila melanogaster/genetics Gene Expression Regulation Genes, Regulator Molecular Sequence Data Regulatory Sequences, Nucleic Acid Salivary Proteins and Peptides/genetics Transcription, Genetic

Chemicals

Chromatin Salivary Proteins and Peptides Deoxyribonuclease I

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Ramain P

Laboratoire de Génétique Moléculaire des Eucaryotes du Centre National de la Recherche Scientifique, Faculté de Médecine, Strasbourg, France.

Giangrande A

Richards G

Bellard M

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23 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.85.8.2718

Published

1988-04-00

Pages

2718-22

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC280070

Subset

External Links

PubMed source DOI: 10.1073/pnas.85.8.2718 PMC Full Text View journal details

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Analysis of a DNase I-hypersensitive site in transgenic Drosophila reveals a key regulatory element of Sgs3.

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