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PMID: 7479023 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cell cycle regulation of cdc25C transcription is mediated by the periodic repression of the glutamine-rich activators NF-Y and Sp1.

Nucleic acids research ·Vol. 23 ·No. 19 ·1995-10-11 ·Pages 3822-30

Zwicker J, Gross C, Lucibello FC, Truss M, Ehlert F, Engeland K, Müller R

Abstract

The late S/G2-specific transcription of the human cdc25C gene is dependent on an initiator-proximal repressor element (CDE) and an upstream activating sequence (UAS) of undefined nature. We now show that these upstream sequences harbour multiple in vivo protein binding sites that interact with transcriptional activators and form separable, context-independent functional modules. Major components of the UAS are a bona fide Sp1 site and three direct sequence repeats (Yc-boxes). The Yc-boxes interact with the CCAAT-box binding protein NF-Y and are critically dependent on synergistic interactions for efficient transcription activation. The NF-Y complexes, as well as Sp1, are constitutive activators, whose activation function is periodically repressed through the CDE. These observations indicate that the cell cycle regulation of cdc25C transcription is mainly due to the CDE-mediated repression of glutamine-rich activators.

MeSH Terms
3T3 Cells Animals Base Sequence Binding Sites CCAAT-Enhancer-Binding Proteins Cell Cycle Cell Cycle Proteins/genetics DNA-Binding Proteins/metabolism Gene Expression Regulation Humans Mice Molecular Sequence Data Phosphoprotein Phosphatases/genetics Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Sp1 Transcription Factor/metabolism Transcription Factors/metabolism cdc25 Phosphatases
Chemicals
CCAAT-Enhancer-Binding Proteins Cell Cycle Proteins DNA-Binding Proteins Sp1 Transcription Factor Transcription Factors Phosphoprotein Phosphatases cdc25 Phosphatases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Zwicker J
Institut für Molekularbiologie und Tumorforschung (IMT), Philipps-Universität Marburg, Germany.
Gross C
Lucibello F C
Truss M
Ehlert F
Engeland K
Müller R
References (37)
37 references, click to expand
  1. A multiplicity of CCAAT box-binding proteins.
    Cell. 1987 Sep 11;50(6):863-72 PMID: 3476205
  2. Mutually exclusive interaction of the CCAAT-binding factor and of a displacement protein with overlapping sequences of a histone gene promoter.
    Cell. 1987 Jul 31;50(3):347-59 PMID: 3607873
  3. A quantitative analysis of nuclear factor I/DNA interactions.
    Nucleic Acids Res. 1988 May 25;16(10):4419-35 PMID: 3380685
  4. A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs.
    Nature. 1988 Jul 21;334(6179):218-24 PMID: 3398920
  5. Analysis of Sp1 in vivo reveals multiple transcriptional domains, including a novel glutamine-rich activation motif.
    Cell. 1988 Dec 2;55(5):887-98 PMID: 3142690
  6. The DNA binding domain of the rat liver nuclear protein C/EBP is bipartite.
    Science. 1989 Mar 31;243(4899):1681-8 PMID: 2494700
  7. Genomic sequencing and methylation analysis by ligation mediated PCR.
    Science. 1989 Nov 10;246(4931):810-3 PMID: 2814502
  8. Luciferase reporter gene vectors for analysis of promoters and enhancers.
    Biotechniques. 1988 May;6(5):454-8 PMID: 2908509
  9. Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in G2.
    Proc Natl Acad Sci U S A. 1990 Jul;87(13):5139-43 PMID: 2195549
  10. A ubiquitous CCAAT factor is required for efficient in vitro transcription from the mouse albumin promoter.
    J Mol Biol. 1990 Aug 20;214(4):865-74 PMID: 2388272
  11. Co-evolution from yeast to mouse: cDNA cloning of the two NF-Y (CP-1/CBF) subunits.
    EMBO J. 1990 Oct;9(10):3119-27 PMID: 1698608
  12. Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells.
    Genes Dev. 1991 Sep;5(9):1538-52 PMID: 1840554
  13. Human SRF-related proteins: DNA-binding properties and potential regulatory targets.
    Genes Dev. 1991 Dec;5(12A):2327-41 PMID: 1748287
  14. The cdc25 M-phase inducer: an unconventional protein phosphatase.
    Cell. 1992 Feb 7;68(3):407-10 PMID: 1310893
  15. Intron-exon organization of the NF-Y genes. Tissue-specific splicing modifies an activation domain.
    J Biol Chem. 1992 May 5;267(13):8984-90 PMID: 1577736
  16. Cell cycle regulation of the human cdc2 gene.
    EMBO J. 1992 May;11(5):1797-804 PMID: 1582409
  17. Three different polypeptides are necessary for DNA binding of the mammalian heteromeric CCAAT binding factor.
    J Biol Chem. 1992 Aug 15;267(23):16574-80 PMID: 1644837
  18. Monoclonal antibodies to NF-Y define its function in MHC class II and albumin gene transcription.
    EMBO J. 1992 Sep;11(9):3315-22 PMID: 1380453
  19. A mammary cell-specific enhancer in mouse mammary tumor virus DNA is composed of multiple regulatory elements including binding sites for CTF/NFI and a novel transcription factor, mammary cell-activating factor.
    Mol Cell Biol. 1992 Nov;12(11):4906-18 PMID: 1328867
  20. An improved thermal cycle for two-step PCR-based targeted mutagenesis.
    Nucleic Acids Res. 1992 Sep 25;20(18):4934 PMID: 1408812
  21. Inducible regulatory elements in the human cyclin D1 promoter.
    Oncogene. 1994 Apr;9(4):1295-304 PMID: 8134134
  22. Structure and cell cycle-regulated transcription of the human cyclin A gene.
    Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5490-4 PMID: 8202514
  23. TBP-TAF complexes: selectivity factors for eukaryotic transcription.
    Curr Opin Cell Biol. 1994 Jun;6(3):403-9 PMID: 7917332
  24. DP and E2F proteins: components of a heterodimeric transcription factor implicated in cell cycle control.
    Curr Opin Cell Biol. 1994 Jun;6(3):443-50 PMID: 7917337
  25. Autoregulatory control of E2F1 expression in response to positive and negative regulators of cell cycle progression.
    Genes Dev. 1994 Jul 1;8(13):1514-25 PMID: 7958836
  26. Multiple DNA elements are required for the growth regulation of the mouse E2F1 promoter.
    Genes Dev. 1994 Jul 1;8(13):1526-37 PMID: 7958837
  27. NF-Y controls transcription of the minute virus of mice P4 promoter through interaction with an unusual binding site.
    J Virol. 1995 Jan;69(1):239-46 PMID: 7983715
  28. Studies on transcription activation by the multimeric CCAAT-binding factor CBF.
    J Biol Chem. 1995 Jan 6;270(1):468-75 PMID: 7814413
  29. Periodic cdc25C transcription is mediated by a novel cell cycle-regulated repressor element (CDE).
    EMBO J. 1995 Jan 3;14(1):132-42 PMID: 7828585
  30. Recombinant rat CBF-C, the third subunit of CBF/NFY, allows formation of a protein-DNA complex with CBF-A and CBF-B and with yeast HAP2 and HAP3.
    Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1624-8 PMID: 7878029
  31. Transcriptional regulation during the mammalian cell cycle.
    Trends Genet. 1995 May;11(5):173-8 PMID: 7785076
  32. Cell cycle regulation of the cyclin A, cdc25C and cdc2 genes is based on a common mechanism of transcriptional repression.
    EMBO J. 1995 Sep 15;14(18):4514-22 PMID: 7556094
  33. THE LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS.
    Exp Cell Res. 1965 Mar;37:614-36 PMID: 14315085
  34. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  35. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  36. A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus.
    Cell. 1985 Jun;41(2):521-30 PMID: 2985280
  37. Human CCAAT-binding proteins have heterologous subunits.
    Cell. 1988 Apr 8;53(1):11-24 PMID: 3349524
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/23.19.3822
Published
1995-10-11
Pages
3822-30
Language
English
Region
England
NLM ID
0411011
PMCID
PMC307297
Subset
IM
Databases
GENBANK
Z29077
External Links
PubMed source DOI: 10.1093/nar/23.19.3822 PMC Full Text View journal details
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