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PMID: 1380453 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Monoclonal antibodies to NF-Y define its function in MHC class II and albumin gene transcription.

The EMBO journal ·Vol. 11 ·No. 9 ·1992-09-00 ·Pages 3315-22

Mantovani R, Pessara U, Tronche F, Li XY, Knapp AM, Pasquali JL, Benoist C, Mathis D

Abstract

NF-Y is a sequence-specific DNA-binding protein which, as a heterodimer, recognizes CCAAT motifs in a variety of transcriptional promoters. We have generated a panel of monoclonal and affinity-purified polyclonal antibodies directed against various epitopes of NF-Y. These reagents are highly specific for either of the A or B subunits; we have mapped the epitopes recognized by the monoclonal antibodies to the glutamine-rich activation domain of NF-YA. The antibodies inhibit in vitro transcription from the promoters of the albumin gene and of Ea, a class II gene of the major histocompatibility complex. These data definitively demonstrate the role of NF-Y in regulating the transcription of two tissue-specific genes whose expression patterns do not overlap. Interestingly, the antibodies cannot inhibit a formed pre-initiation complex, but do block reinitiation of subsequent rounds of transcription from the same templates.

MeSH Terms

Albumins/biosynthesis,genetics Animals Antibodies, Monoclonal/immunology Antibody Affinity Antibody Specificity Base Sequence CCAAT-Enhancer-Binding Proteins Cloning, Molecular DNA-Binding Proteins/immunology,physiology Epitopes Gene Expression Regulation Genes, MHC Class II Mice Mice, Inbred BALB C Models, Genetic Molecular Sequence Data Transcription Factors/immunology,physiology Transcription, Genetic

Chemicals

Albumins Antibodies, Monoclonal CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins Epitopes Transcription Factors

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Mantovani R

Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, France.

Pessara U

Tronche F

Li X Y

Knapp A M

Pasquali J L

Benoist C

Mathis D

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1992-09-00

Pages

3315-22

Language

English

Region

England

NLM ID

8208664

PMCID

PMC556866

Subset

External Links

PubMed source PMC Full Text View journal details

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Monoclonal antibodies to NF-Y define its function in MHC class II and albumin gene transcription.

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