An improved thermal cycle for two-step PCR-based targeted mutagenesis.

Nucleic acids research ·Vol. 20 ·No. 18 ·1992-09-25 ·Pages 4934

Good L, Nazar RN

Abstract

暂无摘要

MeSH Terms

Animals DNA, Ribosomal/genetics Genes, Fungal Mutagenesis, Site-Directed Polymerase Chain Reaction/methods RNA, Ribosomal, 5S/genetics Schizosaccharomyces/genetics Tetrahymena thermophila/genetics

Chemicals

DNA, Ribosomal RNA, Ribosomal, 5S

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Good L

Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada.

Nazar R N

References (5)

5 references, click to expand

A general method for introducing a series of mutations into cloned DNA using the polymerase chain reaction.
Gene. 1991 Jun 15;102(1):67-70 PMID: 1864511
A general and rapid mutagenesis method using polymerase chain reaction.
Gene. 1990 Jul 2;91(1):143-7 PMID: 2169445
Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR).
Nucleic Acids Res. 1989 Jul 11;17(13):5404 PMID: 2548160
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
Science. 1988 Jan 29;239(4839):487-91 PMID: 2448875
A general method for rapid site-directed mutagenesis using the polymerase chain reaction.
Gene. 1990 Nov 30;96(1):125-8 PMID: 2265750

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/20.18.4934

Published

1992-09-25

Pages

4934

Language

English

Region

England

NLM ID

0411011

PMCID

PMC334258

Subset

External Links

PubMed source DOI: 10.1093/nar/20.18.4934 PMC Full Text View journal details

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An improved thermal cycle for two-step PCR-based targeted mutagenesis.

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