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PMID: 7333675 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Purification and partial characterization of a putative precursor to staphylococcal enterotoxin B.

Infection and immunity ·Vol. 34 ·No. 3 ·1981-12-00 ·Pages 900-7

Tweten RK, Iandolo JJ

Abstract

A putative precursor to staphylococcal enterotoxin B (SEB) has been identified as a component of purified membranes from Staphylococcus aureus S6. Agarose gel immunodiffusion analysis of the solubilized membranes demonstrated an immunoreactive protein that formed complete lines of identity with purified extracellular SEB. This putative precursor (pSEB) also had a different electrophoretic mobility from that of extracellular SEB when analyzed by immunoelectrophoresis. When membrane proteins from S6 were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred to nitrocellulose sheets and probed with I-125 labeled, affinity-purified anti-SEB, the pSEB band was identified. The pSEB was approximately 3,500 daltons larger than extracellular SEB. This component was purified by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Two-dimensional peptide maps of the putative SEB precursor revealed that most of the tryptic peptides were identical to those of mature extracellular SEB. When purified membranes of other SEB+ (DU4916 and 10-275) and SEB- (RN450, RN451, S6R, and FR1100) S. aureus strains were analyzed by the nitrocellulose blot procedure, only the SEB+ strains contained this putative SEB precursor on their membranes.

MeSH Terms
Bacterial Proteins/isolation & purification Electrophoresis, Polyacrylamide Gel Enterotoxins/biosynthesis Membrane Proteins/isolation & purification Peptide Fragments/analysis Protein Precursors/isolation & purification Staphylococcus aureus/metabolism
Chemicals
Bacterial Proteins Enterotoxins Membrane Proteins Peptide Fragments Protein Precursors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tweten R K
Iandolo J J
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30 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.34.3.900-907.1981
Published
1981-12-00
Pages
900-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC350954
Subset
IM
Grants
NIAID NIH HHS · AI-17474 · United States
External Links
PubMed source DOI: 10.1128/iai.34.3.900-907.1981 PMC Full Text View journal details
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