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PMID: 6772962 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A signal sequence is not sufficient to lead beta-galactosidase out of the cytoplasm.

Nature ·Vol. 286 ·No. 5771 ·1980-07-24 ·Pages 356-9

Moreno F, Fowler AV, Hall M, Silhavy TJ, Zabin I, Schwartz M

Abstract

Escherichia coli strains have been constructed in which lacZ, the gene for the cytoplasmic enzyme beta-galactosidase, is fused to lamB, the gene for an outer membrane protein. One such strain produces a beta-galactosidase which remains cytoplasmic even though it possesses the complete signal sequence of the lamB protein precursor at the amino-terminal end.

MeSH Terms

Amino Acid Sequence Biological Transport Carrier Proteins/metabolism Cell Membrane/metabolism Cytoplasm/metabolism Escherichia coli/metabolism Galactosidases/metabolism Genetic Engineering Molecular Weight Protein Precursors/metabolism Structure-Activity Relationship beta-Galactosidase/metabolism

Chemicals

Carrier Proteins Protein Precursors Galactosidases beta-Galactosidase

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Moreno F

Fowler A V

Hall M

Silhavy T J

Zabin I

Schwartz M

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/286356a0

Published

1980-07-24

Pages

356-9

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/286356a0 View journal details

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A signal sequence is not sufficient to lead beta-galactosidase out of the cytoplasm.

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