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PMID: 7279665 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequences of Acanthamoeba castellanii 5S and 5.8S ribosomal ribonucleic acids: phylogenetic and comparative structural analyses.

Nucleic acids research ·Vol. 9 ·No. 14 ·1981-07-24 ·Pages 3321-34

MacKay RM, Doolittle WF

Abstract

Sequences of 5S and 5.8S rRNAs of the amoeboid protist Acanthamoeba castellanii have been determined by gel sequencing of terminally-labeled RNAs which were partially degraded with chemical reagents or ribonucleases. The sequence of the 5S rRNA is (formula, see text). This sequence is compared to eukaryotic 5S rRNA sequences previously published and fitted to a secondary structure model which incorporates features of several previously proposed models. All reported eukaryotic 5S rRNAs fit this model. The sequence of the 5.8S rRNA is (formula, see text). This sequence does not fit parts of existing secondary structure models for 5.8S rRNA, and we question the significance of such models.

MeSH Terms
Amoeba/genetics Animals Base Sequence Humans Nucleic Acid Conformation Phylogeny RNA, Ribosomal/genetics Species Specificity Thermodynamics
Chemicals
RNA, Ribosomal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
MacKay R M
Doolittle W F
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37 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.14.3321
Published
1981-07-24
Pages
3321-34
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327354
Subset
IM
Databases
GENBANK
K00471, V00003
External Links
PubMed source DOI: 10.1093/nar/9.14.3321 PMC Full Text View journal details
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