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PMID: 7232217 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence and structure of cytoplasmic 5S RNA and 5.8S RNA of Chlamydomonas reinhardii.

Nucleic acids research ·Vol. 9 ·No. 6 ·1981-03-25 ·Pages 1291-9

Darlix JL, Rochaix JD

Abstract

Three small RNAs of the cytoplasmic 8OS ribosomes of the green unicellular alga Chlamydomonas reinhardii have been sequenced. They include two species of ribosomal 5S RNA, a major and a minor one of 122 and 121 nucleotides respectively, which differ from each other by 17 bases, and also the ribosomal 5.8S RNA of 156 nucleotides. Novel structural features can be recognized in the 5S RNAs of C. reinhardii by a comparison with published 5S RNA sequences. In addition the secondary structure of these small RNA molecules has been examined using a newly developed method based on differential nuclease susceptibility.

MeSH Terms
Base Sequence Chlamydomonas/analysis Cytoplasm/analysis Molecular Weight Nucleic Acid Conformation RNA, Ribosomal Ribonucleases
Chemicals
RNA, Ribosomal Ribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Darlix J L
Rochaix J D
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.6.1291
Published
1981-03-25
Pages
1291-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326757
Subset
IM
Databases
GENBANK
X02706, X02707, X02708
External Links
PubMed source DOI: 10.1093/nar/9.6.1291 PMC Full Text View journal details
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