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PMID: 7443527 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence organization of the repeating units in the nucleus of wheat which contain 5S rRNA genes.

Nucleic acids research ·Vol. 8 ·No. 21 ·1980-11-11 ·Pages 4851-65

Gerlach WL, Dyer TA

Abstract

Examples of both the 410 and 500 bp size classes of repeating units containing wheat 5S rRNA genes have been cloned in plasmid pBR322 and sequenced. The structural genes showed sequence microheterogeneity. Also the gene in the 500 bp repeat which was sequenced had a 15 bp tandem duplication within it and appears to be representative of non-transcribed subfamily of repeating units. The transcription terminators comprise 14-17 A.T bp immediately preceded by a region of weak dyad symmetry. The spacer regions adjacent to the transcription terminators in the two different size repeat units have interspersed oligonucleotides of high and low homology. The central spacer regions of the two size classes have very different sequences. The only repeated sequence in the spacers has undergone extensive divergence. In contrast to most of the spacer, the 70 bp region preceding the genes of each type of repeat show high homology, suggesting that it has functional importance. The transcription start point obeys the pyrimidine-1 purine+1 rule.

MeSH Terms
Base Sequence Biological Evolution Cell Nucleus/physiology Gene Expression Regulation Genes Molecular Weight RNA, Ribosomal/genetics Repetitive Sequences, Nucleic Acid Transcription, Genetic Triticum/genetics
Chemicals
RNA, Ribosomal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gerlach W L
Dyer T A
References (28)
28 references, click to expand
  1. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  2. Endonuclease recognition sites mapped on Zea mays chloroplast DNA.
    Proc Natl Acad Sci U S A. 1976 Dec;73(12):4309-13 PMID: 16592373
  3. Cloned single repeating units of 5S DNA direct accurate transcription of 5S RNA when injected into Xenopus oocytes.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2849-53 PMID: 275856
  4. Nucleotide sequences of chloroplast 5S ribosomal ribonucleic acid in flowering plants.
    Biochem J. 1979 Dec 1;183(3):595-604 PMID: 540034
  5. A general method for the purification of restriction enzymes.
    Nucleic Acids Res. 1978 Jul;5(7):2373-80 PMID: 673857
  6. Is there a correction mechanism in the 5S multigene system?
    Nature. 1978 Oct 12;275(5680):556-8 PMID: 567752
  7. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  8. Cloning and characterization of ribosomal RNA genes from wheat and barley.
    Nucleic Acids Res. 1979 Dec 11;7(7):1869-85 PMID: 537913
  9. A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
    Cell. 1980 Jan;19(1):13-25 PMID: 7357599
  10. Sequence of Drosophila 5S RNA synthesized by cultured cells and by the insect at different developmental stages. Homogeneity of the product and homologies with other 5S RNA's at the level of primary and secondary structure.
    J Mol Evol. 1977 May 13;9(3):279-98 PMID: 864730
  11. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.
    Biochemistry. 1967 Jun;6(6):1818-27 PMID: 6035921
  12. Promotor region for yeast 5S ribosomal RNA.
    Nature. 1977 Jun 16;267(5612):643-5 PMID: 327336
  13. Evidence for the nucleotide sequence of 5-S rRNA from the flowering plant Secale cereale (Rye).
    Eur J Biochem. 1976 Dec;71(1):33-8 PMID: 1009953
  14. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  15. A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
    Cell. 1980 Jan;19(1):27-35 PMID: 7357604
  16. Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit.
    Cell. 1976 Nov;9(3):431-8 PMID: 1033038
  17. Sequence and heterogeneity in the 5S RNA gene cluster of Drosophila melanogaster.
    Nucleic Acids Res. 1980 Feb 11;8(3):441-51 PMID: 6160476
  18. Collection of published 5S and 5.8S rRNA sequences and their precursors.
    Nucleic Acids Res. 1980 Jan 11;8(1):r31-r47 PMID: 6986609
  19. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  20. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
  21. Nucleotide sequence of Xenopus borealis oocyte 5S DNA: comparison of sequences that flank several related eucaryotic genes.
    Cell. 1978 Dec;15(4):1145-56 PMID: 264240
  22. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  23. A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
    Cell. 1978 Nov;15(3):1077-86 PMID: 569551
  24. Nucleotide sequence of the yeast 5S ribosomal RNA gene and adjacent putative control regions.
    Nature. 1977 Jun 16;267(5612):641-3 PMID: 327335
  25. Conformational studies on wheat embryo 5S RNA using nuclease S1 as a probe.
    Can J Biochem. 1978 May;56(5):357-64 PMID: 667686
  26. The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis.
    Cell. 1979 Oct;18(2):485-99 PMID: 498280
  27. Computer analysis of nucleic acid regulatory sequences.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4401-5 PMID: 270683
  28. The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. II. The GC-rich region.
    Cell. 1978 Apr;13(4):717-25 PMID: 566164
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.21.4851
Published
1980-11-11
Pages
4851-65
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324264
Subset
IM
Databases
GENBANK
X06094, X06095
External Links
PubMed source DOI: 10.1093/nar/8.21.4851 PMC Full Text View journal details
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