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PMID: 71978 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The interaction of Hoechst 33258 and BrdU substituted DNA in the formation of sister chromatid exchanges.

Chromosoma ·Vol. 63 ·No. 1 ·1977-08-18 ·Pages 21-31

Stetka DG, Carrano AV

Abstract

Sister chromatid exchanges (SCEs) are induced in cultured Chinese hamster cells by treatment with 5-bromodeoxyuridine (BrdU) or with Hoechst 33258 (H33258) plus BrdU. The SCE frequencies depend upon the number of H33258 molecules available per cell (or per base pair) and the number of brdU molecules available per cell, and not solely upon molarity. In addition, H33258 and BrdU act synergistically to induce SCEs. At low BrdU concentrations H 33258 induces very few SCEs. At high BrdU concentrations and similar concentrations of H33258, however, SCE frequencies are significantly increased. SCE frequencies decrease with time in successively harvested cells because of the depletion of H33258 from the medium due to DNA binding.

MeSH Terms
Benzimidazoles/pharmacology Bisbenzimidazole/pharmacology Bromodeoxyuridine/pharmacology Cells, Cultured Chromatids/drug effects DNA/metabolism Dose-Response Relationship, Drug Drug Synergism Time Factors
Chemicals
Benzimidazoles DNA Bromodeoxyuridine Bisbenzimidazole
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stetka D G
Carrano A V
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23 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00292939
Published
1977-08-18
Pages
21-31
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00292939 View journal details
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