Effect of Hoechst 33258 on Chinese hamster chromosomes.

Chromosoma ·Vol. 56 ·No. 1 ·1976-06-30 ·Pages 41-6

Rocchi A, Prantera G, Pimpinelli S, Di Castro M

Abstract

Cells of the Chinese hamster strain C-125 were treated for different time intervals with H 33258, a bibenzimidazole derivative. The same compound was used to stain fixed cells of the same strain. H 33258 induced in cells in culture specific areas of reduced spiralization on the metaphase chromosomes of some cells. These probably correspond to DNA segments rich in A-T bases interspersed along the chromosomes. Probably H 33258 acts during S period of cell cycle. The banding obtained by staining with H 33258 is similar to that induced by quinacrine dihydrochloride but shows a better resolution.

MeSH Terms

Adenine Nucleotides/analysis Animals Benzimidazoles Bisbenzimidazole Chromosomes/analysis Cricetinae Fluorescent Dyes Mitosis Quinacrine Staining and Labeling Thymine Nucleotides/analysis

Chemicals

Adenine Nucleotides Benzimidazoles Fluorescent Dyes Thymine Nucleotides Quinacrine Bisbenzimidazole

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Rocchi A

Prantera G

Pimpinelli S

Di Castro M

References (14)

14 references, click to expand

Banding patterns of Chinese hamster chromosomes revealed by new techniques.
Chromosoma. 1972;36(3):272-80 PMID: 4112624
Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes.
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3395-9 PMID: 4128545
The sex chromosomes of the Chinese hamster: constitutive heterochromatin deficient in repetitive DNA sequences.
Cytogenet Cell Genet. 1974;13(3):268-74 PMID: 4135468
Replication of repetitious DNA and the S period.
Proc Natl Acad Sci U S A. 1970 Sep;67(1):448-55 PMID: 5272325
Mammalian chromosome identification in interspecific hybride cells using "Hoechst 33258".
Humangenetik. 1975;27(1):9-14 PMID: 49289
Chromosomal localization of mouse satellite DNA.
Science. 1970 Jun 12;168(3937):1356-8 PMID: 5462793
Decondensation of constitutive heterochromatin in L cell chromosomes by a benzimidazole compound ("33258 Hoechst").
Exp Cell Res. 1973 Oct;81(2):474-7 PMID: 4128138
Evidence for heterogeneity in heterochromatin of Drosophila melanogaster.
Nature. 1975 Jul 24;256(5515):335-7 PMID: 806812
Fluorometric properties of the bibenzimidazole derivative Hoechst 33258, a fluorescent probe specific for AT concentration in chromosomal DNA.
Chromosoma. 1974;46(3):255-60 PMID: 4136742
Chromosomal and nuclear location of mouse satellite DNA in individual cells.
Nature. 1970 Mar 7;225(5236):912-5 PMID: 5415126
Distribution of repetitious DNA in randomly growing and synchronized Chinese hamster cells.
Exp Cell Res. 1975 Jan;90(1):79-86 PMID: 1168140
Staining of constitutive heterochromatin in mammalian chromosomes with a new fluorochrome.
Exp Cell Res. 1972 Nov;75(1):122-6 PMID: 4117919
Subunits of chromosomal DNA. I. Electron microscopic analysis of partially denatured DNA.
Chromosoma. 1972;39(3):225-35 PMID: 4634354
DNA of mammalian and avian heterochromatin.
Exp Cell Res. 1972 Mar;71(1):113-31 PMID: 5063568

Article Info

Journal

Chromosoma

Abbr.

Chromosoma

ISSN

0009-5915

DOI

10.1007/BF00293728

Published

1976-06-30

Pages

41-6

Language

English

Region

Austria

NLM ID

2985138R

Subset

External Links

PubMed source DOI: 10.1007/BF00293728 View journal details

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Effect of Hoechst 33258 on Chinese hamster chromosomes.

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