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PMID: 7145704 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effects of DNA replication inhibitors on UV excision repair in synchronised human cells.

Nucleic acids research ·Vol. 10 ·No. 17 ·1982-09-11 ·Pages 5357-68

Downes CS, Collins AR

Abstract

When HeLa cells are irradiated with UV and treated with the DNA synthesis inhibitors hydroxyurea (HU) and 1-beta-D-arabinofuranosylcytosine (ara C), DNA strand breaks accumulate at sites where excision repair of DNA damage has been inhibited after the incision step. This break accumulation occurs in mitotic, G1 and S phase cells. But UV-induced repair synthesis of DNA, as measured by [3H]thymidine incorporation into unreplicated DNA, is not inhibited by HU and ara C in G1 or S phase cells, even though replicative synthesis is virtually abolished. Repair and replication must therefore utilise different DNA precursor pools, or different DNA synthetic systems; and the action of Hu and ara C in causing strand break accumulation may occur at the ligation step of excision repair.

MeSH Terms
Cytarabine/pharmacology DNA Repair DNA Replication/drug effects,radiation effects HeLa Cells/drug effects,metabolism,radiation effects Humans Hydroxyurea/pharmacology Kinetics Ultraviolet Rays
Chemicals
Cytarabine Hydroxyurea
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Downes C S
Collins A R
References (29)
29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.17.5357
Published
1982-09-11
Pages
5357-68
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320877
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.17.5357 PMC Full Text View journal details
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