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PMID: 262382 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA damage in synchronized HeLa cells irradiated with ultraviolet.

Biophysical journal ·Vol. 25 ·No. 1 ·1979-01-00 ·Pages 129-50

Downes CS, Collins AR, Johnson RT

Abstract

The lethal effect of UV radiation of HeLa cells is least in mitosis and greatest in late G1-early S. Photochemical damage to HeLa DNA, as measured by thymine-containing dimer formation and by alkaline sucrose sedimentation, also increases from mitosis towards early S phase. Computer simulations of UV absorption by an idealized HeLa cell at different stages of the cell cycle indicate that changes in damage could be due solely to changes in chromatin geometry. But survival is not exclusively a function of damage.

MeSH Terms
Cell Division/radiation effects Cell Survival/radiation effects Centrifugation, Density Gradient Computers DNA Repair HeLa Cells/radiation effects Humans Models, Biological Ultraviolet Rays
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Downes C S
Collins A R
Johnson R T
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(79)85282-0
Published
1979-01-00
Pages
129-50
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1328452
Subset
IM
External Links
PubMed source DOI: 10.1016/S0006-3495(79)85282-0 PMC Full Text View journal details
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