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PMID: 4626402 Published · ppublish English Journal Article

Chromatin structure and the cell cycle.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 8 ·1972-08-00 ·Pages 2224-8

Pederson T

Abstract

Pancreatic DNase I is used to probe the structure of chromatin isolated from synchronized HeLa cells. The degree to which DNA in chromatin is protected from DNase attack varies during the G(1), S, and G(2) phases of the cell cycle. In addition, the DNase sensitivity of chromatin from contact-inhibited African green monkey kidney cells differs from that of actively dividing, subconfluent cultures. These cell cycle-dependent chromatin changes were observed consistently at all enzyme concentrations (5000-fold range) and incubation times (15 min-2 hr) tested. The results indicate that the degree of complexing between DNA and chromosomal proteins changes during interphase, and they suggest that the chromosome coiling cycle of visible mitosis may extend in more subtle form over the entire cell cycle.

MeSH Terms
Animals Binding Sites Cell Fractionation Cell Line Centrifugation, Density Gradient Chromatin/drug effects,isolation & purification Contact Inhibition DNA/isolation & purification Dactinomycin Deoxyribonucleases/pharmacology Haplorhini HeLa Cells Kidney Methods Mitosis Pancreas/enzymology Thymidine/metabolism Time Factors Tritium
Chemicals
Chromatin Tritium Dactinomycin DNA Deoxyribonucleases Thymidine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Pederson T
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.8.2224
Published
1972-08-00
Pages
2224-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426905
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.8.2224 PMC Full Text View journal details
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