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PMID: 6803229 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inhibitors of repair DNA synthesis.

Nucleic acids research ·Vol. 10 ·No. 4 ·1982-02-25 ·Pages 1203-13

Collins AR, Squires S, Johnson RT

Abstract

We have measured repair DNA synthesis in UV-irradiated normal human fibroblasts, grown to a defined state of quiescence in order to avoid the problem of discriminating repair from replicative DNA synthesis. We have assessed the effects of various DNA synthesis inhibitors on repair. Inhibition of repair synthesis by hydroxyurea, 1-beta-D-arabinofuranosylcytosine and aphidicolin is associated with the ability to accumulate DNA breaks due to enzymic incision at DNA damage sites; the inhibition by novobiocin is in accord with its known ability to block incision.

MeSH Terms

Antibiotics, Antineoplastic/pharmacology Aphidicolin Cell Line Cytarabine/pharmacology DNA Repair DNA Replication/drug effects,radiation effects Diterpenes/pharmacology Dose-Response Relationship, Drug Dose-Response Relationship, Radiation Fibroblasts/radiation effects Humans Hydroxyurea/pharmacology Kinetics Novobiocin/pharmacology Ultraviolet Rays

Chemicals

Antibiotics, Antineoplastic Diterpenes Cytarabine Novobiocin Aphidicolin Hydroxyurea

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Collins A R

Squires S

Johnson R T

References (24)

24 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.4.1203

Published

1982-02-25

Pages

1203-13

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320519

Subset

External Links

PubMed source DOI: 10.1093/nar/10.4.1203 PMC Full Text View journal details

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