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PMID: 7272442 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Application of arabinofuranosyl cytosine in the kinetic analysis and quantitation of DNA repair in human cells after ultraviolet irradiation.

Biophysical journal ·Vol. 35 ·No. 2 ·1981-08-00 ·Pages 339-50

Snyder RD, Carrier WL, Regan JD

Abstract

We have developed a technique whereby 3-h pulses of arabinofuranosyl cytosine (ara-C) and hydroxyurea (HU) are used to analyze the kinetics of repair with time after ultraviolet irradiation in human fibroblasts. We demonstrate that this technique offers a significant improvement over existing repair assays in its ability to visualize between 57 and 100% of all sites undergoing repair in a given period of time. In addition, kinetic analyses of repair are more easily made and yield more information than techniques such as repair replication or unscheduled DNA synthesis. We have also examined the nature of the inhibition event by ara-C and have determined that repair breaks accumulate in the presence of ara-C and HU only up to a certain time beyond which no further breaks appear. The time needed to reach this saturation point depends on the number of sites undergoing repair during the treatment time. This observation is discussed with respect to a possible mechanism of excision repair inhibition by ara-C and HU.

MeSH Terms
Cells, Cultured Cytarabine/pharmacology DNA Repair DNA Replication/drug effects,radiation effects Fibroblasts/metabolism Humans Hydroxyurea/pharmacology Kinetics Skin/metabolism Ultraviolet Rays Xeroderma Pigmentosum/metabolism
Chemicals
Cytarabine Hydroxyurea
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Snyder R D
Carrier W L
Regan J D
References (23)
23 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(81)84793-5
Published
1981-08-00
Pages
339-50
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1327526
Subset
IM
Grants
PHS HHS · 40-5-63 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(81)84793-5 PMC Full Text View journal details
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