Novobiocin; an inhibitor of the repair of UV-induced but not X-ray-induced damage in mammalian cells.

Nucleic acids research ·Vol. 7 ·No. 5 ·1979-11-10 ·Pages 1311-20

Collins A, Johnson R

Abstract

In addition to inhibiting replicative DNA synthesis in HeLa cells, novobiocin has a severe effect on the cellular response to UV irradiation, reducing the number of breaks made in pre-existing DNA by the excision repair process. The inhibition of UV repair by novobiocin is reflected in enhanced UV-killing of these cells. Rejoining of DNA after X irradiation is not impaired by novobiocin. The recognition and removal of UV damage may require unwinding of the DNA by gyrase, which--in bacteria--is the target for novobiocin.

MeSH Terms

DNA Repair/drug effects,radiation effects DNA Replication/drug effects,radiation effects Dose-Response Relationship, Drug Dose-Response Relationship, Radiation HeLa Cells/drug effects,metabolism,radiation effects Humans Kinetics Novobiocin/pharmacology Ultraviolet Rays X-Rays

Chemicals

Novobiocin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Collins A

Johnson R

References (16)

16 references, click to expand

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Single-strand breaks in DNA during repair of UV-induced damage in normal human and xeroderma pigmentosum cells as determined by alkaline DNA unwinding and hydroxylapatite chromatography: effects of hydroxyurea, 5-fluorodeoxyuridine and 1-beta-D-arabinofuranosylcytosine on the kinetics of repair.
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Reversal of the changes in DNA and chromosome structure which follow the inhibition of UV-induced repair in human cells.
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Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.
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Two forms of repair in the DNA of human cells damaged by chemical carcinogens and mutagens.
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/7.5.1311

Published

1979-11-10

Pages

1311-20

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342304

Subset

External Links

PubMed source DOI: 10.1093/nar/7.5.1311 PMC Full Text View journal details

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Novobiocin; an inhibitor of the repair of UV-induced but not X-ray-induced damage in mammalian cells.

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