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PMID: 7122239 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The influence of the purine 2-amino group on DNA conformation and stability. Synthesis and conformational analysis of d[T(2-aminoA)]3.

Nucleic acids research ·Vol. 10 ·No. 14 ·1982-07-24 ·Pages 4351-61

Gaffney BL, Marky LA, Jones RA

Abstract

A self-complementary hexanucleotide consisting of thymidine and 2-amino-deoxyadenosine, d(TA')3, has been synthesized by a solid phase phosphotriester method. Melting studies show that the additional hydrogen bond afforded by the 2-amino group substantially stabilizes the duplex. Moreover, conformational analysis using circular dichroism shows that a salt-induced conformational transition occurs, similar to the B leads to Z transition observed for d(CG)n oligonucleotides.

MeSH Terms
DNA Indicators and Reagents Nucleic Acid Conformation Oligodeoxyribonucleotides/chemical synthesis Oligonucleotides/chemical synthesis Spectrophotometry, Ultraviolet
Chemicals
Indicators and Reagents Oligodeoxyribonucleotides Oligonucleotides DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gaffney B L
Marky L A
Jones R A
References (29)
29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.14.4351
Published
1982-07-24
Pages
4351-61
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320804
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.14.4351 PMC Full Text View journal details
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