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PMID: 7050669 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Developmental regulation of a sporulation-specific enzyme activity in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 2 ·No. 2 ·1982-02-00 ·Pages 171-8

Clancy MJ, Smith LM, Magee PT

Abstract

An alpha-glucosidase activity (SAG) occurs in a/alpha Saccharomyces cerevisiae cells beginning at about 8 to 10 h after the initiation of sporulation. This enzyme is responsible for the rapid degradation of intracellular glycogen which follows the completion of meiosis in these cells. SAG differs from similar activities present in vegetative cells and appears to be a sporulation-specific enzyme. Cells arrested at various stages in sporulation (DNA replication, recombination, meiosis I, and meiosis II) were examined for SAG activity; the results show that SAG appearance depends on DNA synthesis and some recombination events but not on the meiotic divisions.

MeSH Terms
Cycloheximide/pharmacology DNA/biosynthesis Gene Expression Regulation Glucosidases/metabolism Glycogen/metabolism Meiosis Recombination, Genetic Saccharomyces cerevisiae/physiology Spores, Fungal/enzymology alpha-Glucosidases/metabolism
Chemicals
Glycogen DNA Cycloheximide Glucosidases alpha-Glucosidases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Clancy M J
Smith L M
Magee P T
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38 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.2.2.171-178.1982
Published
1982-02-00
Pages
171-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369770
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.2.2.171-178.1982 PMC Full Text View journal details
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