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PMID: 589 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Review

Methods for avoiding proteolytic artefacts in studies of enzymes and other proteins from yeasts.

Methods in cell biology ·Vol. 12 ·1975-00-00 ·Pages 149-84

Pringle JR

Abstract

暂无摘要

MeSH Terms
Aminopeptidases/metabolism Carboxypeptidases/metabolism Cell Fractionation Chloromercuribenzoates/pharmacology Edetic Acid/pharmacology Hexokinase/isolation & purification Hydrogen-Ion Concentration Isoelectric Point Isoflurophate/pharmacology Malate Dehydrogenase/isolation & purification Molecular Weight Pepstatins/pharmacology Peptide Hydrolases/analysis,metabolism Phenanthrolines/pharmacology Phenylmethylsulfonyl Fluoride/pharmacology Phosphofructokinase-1/isolation & purification Protease Inhibitors Protein Denaturation Pyruvate Decarboxylase/isolation & purification Saccharomyces cerevisiae/enzymology Temperature Vacuoles
Chemicals
Chloromercuribenzoates Pepstatins Phenanthrolines Protease Inhibitors Isoflurophate Phenylmethylsulfonyl Fluoride Edetic Acid Malate Dehydrogenase Hexokinase Phosphofructokinase-1 Carboxypeptidases Peptide Hydrolases Aminopeptidases Pyruvate Decarboxylase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Pringle J R
References (9)
9 references, click to expand
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Article Info
Journal
Methods in cell biology
Abbr.
Methods Cell Biol
ISSN
0091-679X
DOI
10.1016/s0091-679x(08)60956-5
Published
1975-00-00
Pages
149-84
Language
English
Region
United States
NLM ID
0373334
Subset
IM
External Links
PubMed source DOI: 10.1016/s0091-679x(08)60956-5 View journal details
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