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PMID: 4604714 Published · ppublish English Journal Article

Macromolecule synthesis and breakdown in relation to sporulation and meiosis in yeast.

Journal of bacteriology ·Vol. 119 ·No. 2 ·1974-08-00 ·Pages 619-28

Hopper AK, Magee PT, Welch SK, Friedman M, Hall BD

Abstract

The time course of synthesis and breakdown of various macromolecules has been compared for sporulating (a/alpha) and nonsporulating (a/a and alpha/alpha) yeast cells transferred to potassium acetate sporulation medium. Both types of cells incorporate label into ribonucleic acid and protein. The gel electrophoresis patterns of proteins synthesized in sporulation medium are identical for sporulating and nonsporulating diploids; both are different from electropherograms of vegetative cells. Sporulating and nonsporulating strains differ with respect to deoxyribonucleic acid synthesis; no deoxyribonucleic acid is synthesized in the latter case, whereas the deoxyribonucleic acid complement is doubled in the former. Glycogen breakdown occurs only in sporulating strains. Breakdown of preexisting vegetative ribonucleic acid and protein molecules occurs much more extensively in sporulating than in nonsporulating cells. A timetable of these data is presented.

MeSH Terms
Adenine/metabolism Autoradiography Chromatography, Thin Layer DNA/biosynthesis,metabolism Electrophoresis, Polyacrylamide Gel Fungal Proteins/biosynthesis,metabolism Glycogen/biosynthesis,metabolism Hydrogen-Ion Concentration Meiosis Methionine/metabolism Oxygen Consumption RNA/biosynthesis,metabolism Saccharomyces cerevisiae/growth & development,metabolism Spores, Fungal/growth & development Sulfur Radioisotopes Time Factors Tritium
Chemicals
Fungal Proteins Sulfur Radioisotopes Tritium RNA Glycogen DNA Methionine Adenine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hopper A K
Magee P T
Welch S K
Friedman M
Hall B D
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.119.2.619-628.1974
Published
1974-08-00
Pages
619-28
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC245649
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.119.2.619-628.1974 PMC Full Text View journal details
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