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PMID: 7050089 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A gene coding for a periplasmic protein is located near the locus for termination of chromosome replication in Escherichia coli.

Journal of bacteriology ·Vol. 151 ·No. 3 ·1982-09-00 ·Pages 1391-6

Harayama S, Hazelbauer GL

Abstract

Hybrid plasmids carrying trg, the genetic locus in closest proximity to terC, coded for several polypeptides in addition to the Trg protein. Polypeptides of 59,000 and 61,000 apparent molecular weight were the most prominent products synthesized in minicells containing the hybrid plasmids. Analysis of the effects of deletions generated by a restriction endonuclease identified a region of DNA immediately adjacent to trg as the putative gene coding for the two polypeptides. Studies with whole cells and minicells showed that the 59,000-dalton polypeptide is a periplasmic protein. Analysis by limited proteolysis indicated that the two polypeptides are related, and a number of observations support the notion that the 61,000-dalton protein is a precursor form of the 59,000-dalton mature exported protein. The identification and characterization of a protein, in addition to Trg, which is produced by a gene in close proximity to terC emphasizes the fact that the region does contain intact and active genes.

MeSH Terms
Bacterial Proteins/genetics Chromosome Mapping Chromosomes, Bacterial/metabolism DNA Replication DNA, Recombinant Escherichia coli/genetics Genes Genes, Bacterial Molecular Weight Plasmids
Chemicals
Bacterial Proteins DNA, Recombinant
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Harayama S
Hazelbauer G L
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24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.151.3.1391-1396.1982
Published
1982-09-00
Pages
1391-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC220419
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.151.3.1391-1396.1982 PMC Full Text View journal details
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